KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/11325
Title: | Isolation, Purification and Immobilization of sulfide oxidase from alcaligenes faecalis |
Other Titles: | Not Available |
Authors: | D. Thulasi R.Saraswathy M. Muralidhar |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR - Central Institute of Brackishwater Aquaculturre |
Published/ Complete Date: | 2017-08-01 |
Project Code: | Not Available |
Keywords: | Sulfde, Alcaligenes faecalis, sulfde oxidase, chitosan, immobilization |
Publisher: | CIBA |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | Sulfde oxidase is the enzyme involved in sulfde pathway which catalyzes the conversion of toxic form of sulfde to non toxic sulfate. In this study, an attempt was made to isolate and purify sulfde oxidase from Alcaligenes faecalis and its activity was measured. The isolated enzyme was purifed using acetone precipitation and ion exchange chromatography. Column purifed extracts registered sulfde oxidase activity of 39 µm/hr/mg of protein and the purifcation was increased up to 65 fold. The optimum sulfde oxidase activity was found in pH (6- 8), salinity (10-30 ppt) and temperature (30 & 35 ◦C). In addition to this, chitosan beads were prepared for the immobilization of sulfde oxidase. Further, experiments to evaluate the capacity of the immobilized enzyme to remove sulfde with more emphasis on stability in field conditions would provide useful information to mitigate sulfde from aquaculture ponds |
Description: | Not Available |
ISSN: | 2249-555X |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Indian Journal of Applied Research |
Volume No.: | 7 (8) |
Page Number: | 139-141 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/Publication/handle/123456789/11325 |
Appears in Collections: | FS-CIBA-Publication |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
isolation, purificationand immobilization of sulphide oxidase.PDF | 675.53 kB | Adobe PDF | View/Open |
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.