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http://krishi.icar.gov.in/jspui/handle/123456789/11483
Title: | Rachilla: A novel source for embryogenic callus induction and transformation in rice. |
Other Titles: | Not Available |
Authors: | Visarada Sailaja M Arti P Balachandran SM NP Sarma |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Indian Institute of Millets Research ICAR::Indian Institute of Rice Research |
Published/ Complete Date: | 1998-01-01 |
Project Code: | Not Available |
Keywords: | rice tissue culture embryogenic panicle regeneration |
Publisher: | Not Available |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | Rachilla, the portion joining sterile glumes to palea and lemma, in the rice spikelet has been found to be an additional source of embryogenic callus and transformation. Induction of callus from rachilla was distinct in inflorescences of size 3 cm or above. Inflorescence length ranging between 3 and 5 cm was found to be ideal for induction of callus from rachilla. The response was optimum when the florets were of 2-5 mm. The callus induced from rachilla is lustrous, friable and embryogenic, and facilitated much faster establishment of uniform embryogenic suspension cultures (3-4 weeks) compared to seed-derived calli (6-8 weeks). Though the frequency of callus induction from rachilla is low compared to mature seed-derived calli, the regeneration potential of the former is higher. The rachilla callus could be used for bombardment either directly or after establishment of suspension cultures. The calli derived from rachilla did show advantage over seed-derived calli by hastening the process of establishing suspension cultures or for genetic transformation. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Journal |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Current Science |
NAAS Rating: | 6.73 |
Volume No.: | 74 |
Page Number: | 1004-1008 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/Publication/handle/123456789/11483 |
Appears in Collections: | CS-IIMilletsR-Publication |
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