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http://krishi.icar.gov.in/jspui/handle/123456789/14067
Title: | Molecular characterization of wilt resistant and susceptible genotypes of Cajanus cajan (L.) |
Other Titles: | Not Available |
Authors: | Rakesh M. Swami, Mahesh K. Mahatma, Mithil J. Parekh and V. Khandelwal |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR-Directorate of Groundnut Research |
Published/ Complete Date: | 2015-04-20 |
Project Code: | Not Available |
Keywords: | ISSR, Pigeonpea, RAPD, SSR, Wilt |
Publisher: | AGRICULTURAL RESEARCH COMMUNICATION CENTRE |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | Characterization of germplasm is important for breeding programme. Therefore, in present investigation 9 genotypes of pigeonpea [Cajanuscajan (L) Millsp] comprising three wilt resistant genotypes (Vaishali, ICPL 87119 and national check ICP 8863) and two susceptible genotypes (T15-15 and national check ICP 2376) along with four other popular genotypes grown in Gujarat, India (BDN-2, GT-100, GT-102 and C-11) were characterized using RAPD, ISSR and SSR markers. Genetic diversity of 9 pigeonpea genotypes revealed an average polymorphism of 39.18% by RAPD, 47.67 % by ISSR and 57.35 % by SSR. On the basis of polymorphism (%), PIC(polymorphic information content) value, MI (Marker index) value primer OPI-12 was found most informative among 12 RAPD primers. While ISSR primer UBC-850 was found most informative among 8 ISSR primers showing 80% polymorphism, 0.51 PIC value and 40.80 MI value. SSR primers gave 2 to 5 amplicon on a 6 % polyacrylamide gel. The average PIC value was 0.36, ranging from 0 to 0.73. PIC also provides the estimate of discriminatory power of a locus in the case of SSR marker system by taking into account not only the number of alleles expressed, but also the relative frequency of those alleles. Our prime target of discrimination between wilt resistant and susceptible genotypes of pigeon pea was achieved by ISSR and SSR markers. However, SSR was more efficient in generation of polymorphism among the genotypes based on the information on the level of polymorphism and the PIC value. |
Description: | Not Available |
ISSN: | Print ISSN:0250-5371 Online ISSN:0976-0571 |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Legume Research |
NAAS Rating: | 6.53 |
Volume No.: | 38(6) |
Page Number: | 726-733 |
Name of the Division/Regional Station: | Crop sicence |
Source, DOI or any other URL: | DOI: 10.18805/lr.v38i6.6715 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/14067 |
Appears in Collections: | CS-DGR-Publication |
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