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http://krishi.icar.gov.in/jspui/handle/123456789/18053
Title: | Molecular cloning and in silico analysis of DREB-like gene in watermelon |
Other Titles: | Chet Ram , Manjusha Verma, Bhupendra Singh Panwar and Lalit Arya |
Authors: | Not Available |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR:Central Institute for Arid Horticulture |
Published/ Complete Date: | 2015-09-01 |
Project Code: | Not Available |
Keywords: | Drought tolerance DREB in silico analysis watermelon |
Publisher: | Indian Society of Horticulture New Delhi |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | The objective of this study was to identify and characterize sequences from watermelon related to drought tolerant genes. In order to achieve this, publicly available Cucumis melo REB gene (Cme-DREB1) sequence was retrieved from NCBI database and gene-specific primers were synthesized. A fragment of 274 bp corresponding to DREB-like gene was amplified from genomic DNA of watermelon. PCR product was cloned in E. coli vector (pDrive) and validated through sequencing. The BLAST search showed 99 and 97% homology to CmeDREB1 gene and C. sativus DREB3-like (CsDREB3-like) gene, respectively. Furthermore, it showed 87 to 93% homology with rest of DREB genes available in the public database. Full length watermelon DREB-like gene (C. lanatus DREB-like, ClaDREB-like) was identified from cucurbit genomic database and investigated through in silico analysis. The intronless structural organization of ClaDREB-like gene was found with a conserved AP2/ERF domain. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Article |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Indian Journal of Horticulture |
NAAS Rating: | 6.16 |
Volume No.: | 72(3) |
Page Number: | 2015: 338-342 |
Name of the Division/Regional Station: | Division of Crop Improvement |
Source, DOI or any other URL: | DOI : 10.5958/0974-0112.2015.00066.3 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/18053 |
Appears in Collections: | HS-CIAH-Publication |
Files in This Item:
File | Description | Size | Format | |
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Molecular cloning and in silico analysis of DREB-like gene in watermelon.pdf | 413.83 kB | Adobe PDF | View/Open |
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