KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/19116
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sunita Singh | en_US |
dc.contributor.author | Sukanta Dash | en_US |
dc.contributor.author | K. Rinu | en_US |
dc.contributor.author | S. Saha | en_US |
dc.contributor.author | S. Gupta | en_US |
dc.contributor.author | S. Mandjiny | en_US |
dc.contributor.author | D. Upadhyay | en_US |
dc.contributor.author | L Holmes | en_US |
dc.date.accessioned | 2019-05-02T05:34:21Z | - |
dc.date.available | 2019-05-02T05:34:21Z | - |
dc.date.issued | 2016-01-01 | - |
dc.identifier.citation | Not Available | en_US |
dc.identifier.issn | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/19116 | - |
dc.description | Not Available | en_US |
dc.description.abstract | The facultative anaerobe, Lactococcus lactis was grown in batch fermentation (1.5 L working volume) at 30°C, using 5% inoculums to initiate growth for the production of the antimicrobial food additive nisin. L. lactis strain NCIM 2114 produced a maximal nisin concentration of 50,400 IU/mL and 8,830 IU/mg biomass in MRS growth medium using a 2 L bioreactor with optimal conditions of 25 rpm and 0.2 vvm determined by a Box Behnken design statistical package. Nisin was obtained from cell-free extracts and quantified using ultra-fast liquid chromatography (UFLC). Maximum bioactive nisin concentration was found at 4 h and harvested. Increasing aeration, agitation or time until harvest had negative impact on bioactive product yield. In a repeated trial under the same conditions, cell-free extracts of nisin were measured by well diffusion assay at 240 IU/mL (antimicrobial activity). Bioactive nisin was confirmed. The optimal specific growth rate for L. lactis was measured to be 0.25 h-1. This study shows that active biomass production can be increased using conditions determined by standard statistical methods. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Not Available | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Nisin | en_US |
dc.subject | Food preservative | en_US |
dc.subject | RP-UFLC | en_US |
dc.subject | Fermentation technology | en_US |
dc.subject | Bioreactor, Bioactivity | en_US |
dc.subject | Cell-free extract | en_US |
dc.subject | Box Behnken | en_US |
dc.title | Nisin Production in a Two Liters Bioreactor Using Lactococcus lactis NCIM 2114 | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Journal of Medical and Biological Science Research | en_US |
dc.publication.volumeno | 2(2) | en_US |
dc.publication.pagenumber | 21-26 | en_US |
dc.publication.divisionUnit | Design of Experiment | en_US |
dc.publication.authorAffiliation | ICAR::Indian Agricultural Research Institute | en_US |
dc.publication.authorAffiliation | ICAR::Indian Agricultural Statistics Research Institute | en_US |
dc.publication.authorAffiliation | Sartorius Stedim Biotechnology Research and Training Center, Department of Chemistry and Physics, PO Box 1510, Pembroke. | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.naasrating | Not Available | - |
Appears in Collections: | AEdu-IASRI-Publication |
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