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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/2109
Title: | Superoxide Dismutase Isozymes and Their Heat Stability in Coconut (Cocus nucefera L.) Leaves |
Other Titles: | Not Available |
Authors: | Mukesh Kumar, Sugatha P. and K.B. Hebbar |
Published/ Complete Date: | 2014-04-01 |
Project Code: | Not Available |
Keywords: | Not Available SOD activity, Heat stability, coconut, isoforms |
Publisher: | Not Available |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | The possible isoforms of superoxide dismutase (SOD: EC 1.15.1.1) and its stability against heat treatment at various temperatures (500 C to 1000 C) in the three year old seedlings of West Coast Tall (WCT) cultivar of coconut were investigated. To identify SOD activity, crude extract from coconut leaves were subjected to native polyacrylamide gel electrophoresis followed by staining with nitroblue tetrazolium (NBT) and riboflavin. From our study, we found out 14 isoforms for superoxide dismutases in coconut. Treatment with SOD inhibitors indicated the presence of five Cu/Zn-SOD, five Mn- SOD and two Fe-SODS. In this experiment, we also found the presence of two higher molecular weight SOD isoforms, which were resistant to both the SOD inhibition treatments (Cu/Zn-SOD, Fe-SOD and Mn-SOD, Fe-SOD inhibitors). These two SOD isoforms were unstable to heat treatment and completely lost their activity by 600 C. Under sequential heat treatments SOD specific activity was decreased linearly from 6.82 to 1.88 till 800 C and increased at 100 0C. This may be due to the activation of some new SOD isoforms at higher temperature. Studies on the effect of sequential heat on isoforms revealed that, out of 14 isoforms, ten isoforms lost their activity at 800 C and only 4 isoforms (two each for Cu/Zn-SOD and Mn-SOD) were stable at 800 C, and at 1000 C only two existing isoforms of Cu/Zn-SOD were stable but two new isoforms of Cu/Zn-SODs were also reappeared. |
Description: | Not Available |
ISSN: | 0970-0153 |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Annals of Biology |
NAAS Rating: | 3.95 |
Volume No.: | 30 |
Page Number: | 593-597 |
Name of the Division/Regional Station: | Crop Improvement |
Source, DOI or any other URL: | https://www.cabdirect.org/cabdirect/FullTextPDF/2015/20153005665.pdf |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/2109 |
Appears in Collections: | HS-CIAH-Publication |
Files in This Item:
File | Description | Size | Format | |
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SOD isozyme and their heat stability in Coconut leaves.pdf | 343.65 kB | Adobe PDF | View/Open |
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