KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/21477
Title: | CRISPR/Cas9 Mediated Genome Engineering for Improvement of Horticultural Crops |
Authors: | Karkute S.G., Singh A.K., Gupta O.P., Singh P.M. and Singh B. |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Indian Institute of Vegetable Research ICAR::Indian Institute of Wheat and Barley Research |
Published/ Complete Date: | 2017-09-22 |
Project Code: | Not Available |
Keywords: | CRISPR/Cas9, genome editing, vegetables, fruits, horticultural crops |
Publisher: | Frontiers in Plant Science |
Citation: | Karkute SG, Singh AK, Gupta OP, Singh PM and Singh B (2017) CRISPR/Cas9 Mediated Genome Engineering for Improvement of Horticultural Crops. Front. Plant Sci. 8:1635. doi: 10.3389/fpls.2017.01635 |
Series/Report no.: | Not Available; |
Abstract/Description: | Horticultural crops are an important part of agriculture for food as well as nutritional security. However, several pests and diseases along with adverse abiotic environmental factors pose a severe threat to these crops by affecting their quality and productivity. This warrants the effective and accelerated breeding programs by utilizing innovative biotechnological tools that can tackle aforementioned issues. The recent technique of genome editing by Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated 9 (CRISPR/Cas9) has greatly advanced the breeding for crop improvement due to its simplicity and high efficiency over other nucleases such as Zinc Finger Nucleases and Transcription Activator Like Effector Nucleases. CRISPR/Cas9 tool contains a non-specific Cas9 nuclease and a single guide RNA that directs Cas9 to the specific genomic location creating double-strand breaks and subsequent repair process creates insertion or deletion mutations. This is currently the widely adopted tool for reverse genetics, and crop improvement in large number of agricultural crops. The use of CRISPR/Cas9 in horticultural crops is limited to few crops due to lack of availability of regeneration protocols and sufficient sequence information in many horticultural crops. In this review, the present status of applicability of CRISPR/Cas9 in horticultural crops was discussed along with the challenges and future potential for possible improvement of these crops for their yield, quality, and resistance to biotic and abiotic stress |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Article |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Frontiers in Plant Science |
NAAS Rating: | 10.4 |
Volume No.: | Not Available |
Page Number: | Not Available |
Name of the Division/Regional Station: | Vegetable Improvement |
Source, DOI or any other URL: | https://doi.org/10.3389/fpls.2017.01635 https://www.frontiersin.org/articles/10.3389/fpls.2017.01635/full |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/21477 |
Appears in Collections: | HS-IIVR-Publication |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
fpls-08-01635.pdf | 226.75 kB | Adobe PDF | View/Open |
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.