KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/25187
Title: | Isolation, purification and characterization of proteoglycans from deep sea shark, Echinorhinus brucus (Bramble shark) cartilage |
Other Titles: | Not Available |
Authors: | Ajeeshkumar, K. K. Vishnu, K.V. Asha, K.K. Remyakumari, K. R. Navneethan, R. Shyni, K. Chatterjee, N.S. Suseela Mathew |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Central Institute of Fisheries Technology |
Published/ Complete Date: | 2017-11-21 |
Project Code: | Not Available |
Keywords: | Not Available |
Publisher: | ICAR-Central Institute of Fisheries Technology, Kochi and Asian Fisheries Society, Indian Branch |
Citation: | Ajeeshkumar, K. K.,Vishnu, K.V.,Asha, K.K.,Remyakumari, K. R.,Navneethan, R.,Shyni, K.,Chatterjee, N.S. and Suseela Mathew (2017) Isolation, purification and characterization of proteoglycans from deep sea shark, Echinorhinus brucus (Bramble shark) cartilage. In: (Thomas, S.N., Rao, B.M., Madhu, V.R., Asha, K.K., Binsi, P.K., Viji, P., Sajesh, V.K. and Jha, P.N., Eds.) Fostering Innovations in Fisheries and Aquaculture: Focus on Sustainability and Safety – Book of Abstracts, 11th Indian Fisheries and Aquaculture Forum, ICAR-Central Institute of Fisheries Technology, Kochi and Asian Fisheries Society, Indian Branch, 21-24 November, 2017, Kochi, India, pp. 514-515. |
Series/Report no.: | Not Available; |
Abstract/Description: | The present study was aimed at isolation of proteoglycans from deep sea shark cartilage, Echinorhinus brucus and characterization of their glycosaminoglycans and protein. PGs are important macromolecules of extracellular matrix involved in various cellular functions including cell growth and differentiation. PGs were isolated by chaeotropic digestion and anion-exchange chromatography with DEAEsephacel. Chemical composition such as protein and GAGs were determined by colorimetric assays. SDS-PAGE and chromatographic methods were implemented for further characterization. Trypsin digested samples were used to characterize the protein by peptide based LC-MS/MS analysis. Sample was injected into Q Exactive Plus Bench top Orbitrap instrument coupled to Thermo Easy nLC 1200. The acquired data was analysed in Proteome Discoverer 2.1SP1 on Sequest HT search engine and NCBI full protein database. GAG characterization was done with positive and negative ion full scanning of sample and standard with aid of MS/MS QTRAP 4000 model. NCBI protein data base revealed the presence of aggrecan core protein, decorin and epiphycan peptide hits in the proteoglycans sample and similar ion patterns were noticed for GAG isolated from sample compared to standard GAGs. In chemical composition, total protein, total carbohydrate, uronic acid, hexosamine and sulfated GAGs values were observed to be 0.468 mg/mg, 0.530 mg/mg, 0.098 mg/mg, 0.20 mg/mg and 0.06 mg/mg, respectively. According to the results, it was clear that in AGE both high molecular and low molecular weight PG were present in the isolated sample. Presence of GAGs was confirmed by PAGE using standard GAGs. These methods can be used to characterize the chemical and structural characterization of proteoglycans. In conclusion PGs isolated in our study were found to be pure as confirmed by structural and chemical characterization. |
Description: | Not Available |
ISBN: | 978-81-933623-1-0 |
Type(s) of content: | Other |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Not Available |
Volume No.: | Not Available |
Page Number: | 514-515 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/25187 |
Appears in Collections: | FS-CIFT-Publication |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
514_Isolation purification and characterization.pdf | 185.74 kB | Adobe PDF | View/Open |
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.