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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/25508
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jayanthi. M, Murali Mohan N., Mandal P.K. | en_US |
dc.date.accessioned | 2019-11-27T05:39:01Z | - |
dc.date.available | 2019-11-27T05:39:01Z | - |
dc.date.issued | 2011-07-11 | - |
dc.identifier.citation | Not Available | en_US |
dc.identifier.issn | 0971-7811 | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/25508 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Direct embryogenesis without an intervening callus phase from cotyledonary nodes of germinated immature zygotic embryos of hybrids viz. DG1 and DG21 of oil palm (Elaeis guineensis) is reported here. Direct embryogenesis was achieved when the cotyledonary nodes of germinated immature zygotic embryos were cultured in dark for 8 weeks on Eeuwens media (Y3) supplemented with 40 μM 2,4-Dichlorophenoxyacetic acid (2,4-D), 40 μM α-Naphthaleneacetic acid (NAA), 10 μM 2,4,5-Trichorophenoxyacetic acid (2,4,5-T), 10 μM Thiadiazuron (TDZ), 10 μM 6-Benzyladenine (BA). The globular embryos with clear suspensor region appeared directly on the explants and multiplied. On subculture to fresh media, the other stages such as torpedo and heart shaped embryos were seen. On transfer to light in Y3 media containing BA (2 μM) and ABA (1 μM) they matured into complete plantlets. In 2% of the cultures secondary embryogenesis also was seen. Along with several other advantages of direct somatic embryogenesis this protocol opens up the prospect of genetic transformation in this important commercial crop. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Not Available | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Oil palm In vitro Direct somatic embryogenesis | en_US |
dc.title | Direct embryogenesis and plantlet regeneration in oil palm. | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Journal of Plant Biochemistry and Biotechnology | en_US |
dc.publication.volumeno | 20(2) | en_US |
dc.publication.pagenumber | Not Available | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | Not Available | en_US |
dc.publication.authorAffiliation | Directorate of Oil Palm Research Pedavegi India | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.naasrating | 6.77 | en_US |
Appears in Collections: | CS-NIPB-Publication |
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