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http://krishi.icar.gov.in/jspui/handle/123456789/26478
Title: | Isolation and expression analyses of KLUH gene in developing seeds and enhanced seed oil in KLUH overexpressing Brassica juncea transgenics |
Other Titles: | Not Available |
Authors: | Siddanna Savadi, Deepak Singh Bisht, Shripad Ramachandra Bhat |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::National Institute for Plant Biotechnology |
Published/ Complete Date: | 2017-09-26 |
Project Code: | Not Available |
Keywords: | fatty acid; seed coat; KLUH; gene overexpression |
Publisher: | De Gruyter |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | Improving oil yield of Indian mustard (Brassica juncea) is exigent as it is a major oilseed crop of Indian subcontinent, which has severe shortage of vegetable oil production in the world. Some of the regulators of seed development have been shown to improve oil yield in Arabidopsis. Arabidopsis KLUH (AtKLUH), a maternal regulator of seed size, has been shown to control seed oil content. In this study, we identified three homologs of AtKLUH in B. juncea, BjKLUH1, BjKLUH2-1 and BjKLUH2-2. We observed that BjKLUH1 differentially expresses in developing seeds in B. juncea accessions with varying seed size and oil content. Further, analyses for seed oil content in B. juncea transgenics carrying AtKLUH demonstrated an increase in seed oil up to 8.3% compared to wild-type plants. The results of this study suggest that KLUH may have a role in seed development and is a good candidate for engineering seed oil accumulation in B. juncea. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Article |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Biologia |
NAAS Rating: | 6.81 |
Volume No.: | 72 |
Page Number: | 1023-1030 |
Name of the Division/Regional Station: | Molecular Biology and Biotechnology |
Source, DOI or any other URL: | https://doi.org/10.1515/biolog-2017-0123 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/26478 |
Appears in Collections: | HS-DCR-Publication |
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