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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/2908
Title: | Assessing the colonizing ability of different isolates of Trichoderma spp in castor roots |
Project Code: | OXX02190 |
Published/ Complete Date: | 2016-03-31 |
Division or Unit: | CROP IMPROVEMENT |
PI Name: | V. Dinesh Kumar |
Project Investigator Designation: | Principal Scientist (Biotechnology) |
Project Investigator Email: | vdinesh.kumar@icar.gov.in |
CoPI Name: | R.D. Prasad S. Senthilvel Ch. Sarada T.P. Sujatha |
Co-Project Investigator Designation: | Principal Scientist Senior Scientist Principal Scientist Senior Scientist |
Co-Project Investigator Email: | ravulapalliprasad@gmail.com senthilvel.senapathy@icar.gov.in c.sarada@icar.gov.in sujatha.parvathy@icar.gov.in |
Co-Center PI/Any Collaborator: | Not Available |
Co-Center PI/Any Collaborator Designation: | Not Available |
Co-Center PI/Any Collaborator Email: | Not Available |
Current Location of Data : | With PI of the project |
Current Person's Email: | vdinesh.kumar@icar.gov.in |
Since when: | 2015-04-01 |
Abstract/Description: | As the Trichoderma strain Th4d was identified to be the best in colonizing castor roots (genotype DCS107) through microscopic and plating techniques, more directed efforts were made towards identifying the presence of Trichoderma in castor roots in situ through specific staining of fungal chitin with wheat germ agglutinin – Fluorescent iosthiocyanate (WGA-FITC) conjugate and visualization of the fungus using fluorescence microscopy. Castor seeds were treated with 107 spores/ ml of Th4d for 24 hrs and sown in sterilized soil and those seedlings were used for the present study. Also, Th4d treated and untreated roots of 12-day old castor seedlings in hydroponics were collected at different time points (0, 24, 48, 72 and 96 hpi) and from 35 day-old potted plants (seed treatment with Th4D). Longer staining duration resulted in better staining and even very long duration (e.g., overnight) did not cause a significant increase in background. Good resolution Trichoderma fungal structures were observed(Fig. 1 A-D). To study colonization and multiplication of Th4D in castor roots at different time-points, scanning electron microscopy of roots colonized with Th4D at 6, 12, 24 and 48 hpi was performed. It was observed that multiplication of the fungus increased with time and penetration of the fungal hyphae through the interstitial spaces of the root epidermal cells was observed very clearly by SEM (Fig.2) and these results were reconfirmed using TEM (Fig. 3) |
Funding Type: | External |
Funding Source: | NASF |
Subject: | Agriculture Biotechnology |
Keywords: | Root colonization by Trichoderma flourescent microscopy Transmission electron microscopy Scanning electron microscopy |
List of Attributes: | Images colony enumeration |
Available Data Format: | Other |
Data Scale: | Not Available |
Language: | English |
Extent Time To: | 1001-01-01 |
Extent Time From: | 1001-01-01 |
Availability (Extent): | Not Available |
Data Location: | Project Team |
Source: | Not Available |
Relation: | Colonization images |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/2908 |
Appears in Collections: | CS-IIOR-Data Inventory |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Root Colonization images.pdf | 380.29 kB | Adobe PDF | View/Open |
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