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http://krishi.icar.gov.in/jspui/handle/123456789/31640
Title: | Prediction and expression analysis of microRNAs associated with heat stress in Oryza sativa |
Other Titles: | Not Available |
Authors: | Sailaja B, Voleti SR, Subrahmanyam D, Sarla N, Vishnu Prasanth V, Bhadana VP, and Mangrauthia SK |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR-Indian Institute of Rice Research Hyderabad |
Published/ Complete Date: | 2014-09-01 |
Project Code: | Not Available |
Keywords: | miRNA Oryza sativa Arabidopsis target mRNA regulation |
Publisher: | Elsevier |
Citation: | Sailaja B, Voleti SR, Subrahmanyam D, Sarla N, Vishnu Prasanth V, Bhadana VP, and Mangrauthia SK* (2014). Prediction and expression analysis of microRNAs associated with heat stress in Oryza sativa. Rice Science, 21:3-12. |
Series/Report no.: | Not Available; |
Abstract/Description: | Computational prediction of potential microRNAs (miRNAs) and their target genes was performed to identify the miRNAs and genes associated with temperature response in rice. The data of temperature-responsive miRNAs of Arabidopsis, and miRNAs and the whole genome data of rice were used to predict potential miRNAs in Oryza sativa involved in temperature response. A total of 55 miRNAs were common in both the species, and 27 miRNAs were predicted at the first time in rice. Target genes were searched for these 27 miRNAs in rice genome following stringent criteria. Real time PCR based on expression analysis of nine miRNAs showed that majority of the miRNAs were down regulated under heat stress for rice cultivar Nagina 22. Furthermore, miR169, miR1884 and miR160 showed differential expression in root and shoot tissues of rice. Identification and expression studies of miRNAs during heat stress will advance the understanding of gene regulation under stress in rice. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Rice Science |
Volume No.: | 21 |
Page Number: | 3-12 |
Name of the Division/Regional Station: | Biotechnology |
Source, DOI or any other URL: | https://doi.org/10.1016/S1672-6308(13)60164-X |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/31640 |
Appears in Collections: | CS-IIRR-Publication |
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