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http://krishi.icar.gov.in/jspui/handle/123456789/33410
Title: | Regeneration and evaluation of somaclones of sugarcane variety Co86032 for yellow leaf disease resistance and yield traits |
Other Titles: | Not Available |
Authors: | PRAVEEN KONA, M HEMANTH KUMAR, K H P REDDY, T M HEMALATHA, D M REDDY, N P ESWAR REDDY and P LATHA |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Directorate of Groundnut Research Agricultural Research Station, Perumallapalle, India Department of Genetics and Plant Breeding, S.V. Agricultural College, Tirupati, India Institute of Frontier Technologies, RARS, Tirupati, India |
Published/ Complete Date: | 2019-04-08 |
Project Code: | Not Available |
Keywords: | RT-PCR somaclones sugarcane YLD |
Publisher: | Indian Academy of Sciences, Springer Link |
Series/Report no.: | Not Available; |
Abstract/Description: | The present investigation was focussed on regeneration, evaluation and screening of somaclones for yellow leaf disease (YLD) resistance using in vitro mutagenesis from a popular susceptible sugarcane variety Co86032 using four chemical mutagens at three levels of concentration (sodium azide (SA) at 0.5 mg L-1 , 1.0 mg L-1 , 1.5 mg L-1 ; sodium nitrite (SN) at 3 mg L-1, 5 mg L-1, 7 mg L-1 ; ethyl methane sulphonate (EMS) at 0.6 lM L-1, 0.8 lM L-1, 1.0 lM L-1 and 2,4 D at 4 mg L-1, 5 mg L-1, 6 mg L-1). A total of 1138 tissue culture seedlings obtained were evaluated for virus resistance both in natural field conditions and in controlled greenhouse condition after aphid vector transmission and presence or absence of virus was observed by visual screening and reverse transcription-polymerase chain reaction method. Four out of 207 asymptomatic plants (16T22, 16T23, 16T29 and 16T31) were devoid of virus coat protein band and were considered to be YLD resistant. The obtained resistance somaclones showed inferior yield traits so they have to be exploited as parents in hybridization programmes with commercial varieties to impart YLD resistance ultimately yielding agronomically superior YLD-resistant varieties in sugarcane. |
Description: | Ph.D thesis work. |
ISSN: | Not Available |
Type(s) of content: | Article |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Journal of Biosciences (Proceedings of Indian Academy of Sciences) |
NAAS Rating: | 7.65 |
Volume No.: | 44(29) |
Page Number: | 1-9 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | 10.1007/s12038-019-9846-x |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/33410 |
Appears in Collections: | CS-DGR-Publication |
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