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http://krishi.icar.gov.in/jspui/handle/123456789/33412
Title: | Isolation and Characterization of Inositol Tetraphosphate 1-Kinase (AhITPK1) and Inositol 1,4,5-Tris-Phosphate Kinase (AhIPK2) Gene in Peanut |
Other Titles: | Not Available |
Authors: | Ajay BC, Ananga A, Kambiranda D, Obuya J, Bera SK, and Tsolova V |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Directorate of Groundnut Research Florida A&M University |
Published/ Complete Date: | 1001-01-01 |
Project Code: | Borlaug Fellowship |
Keywords: | Arachis hypogaea; Gene model; Multiple sequence alignment; Inositol tetraphosphate 1-kinase (ITPK1); Inositol 1,4,5-trisphosphate kinase (IPK2); Phylogenetic analysis; Real time PCR |
Publisher: | Not Available |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | In the current study, partial cDNA clones of inositol tetraphosphate 1-kinase (ITPK1) and inositol 1,4,5-trisphosphate kinase/inositol polyphosphate multikinase (IPK2), were isolated from embryo using RT-PCR and designated as AhITPK1 and AhIPK2 isoforms of the gene. The partial cDNA sequence of AhITPK1 and AhIPK2 genes have an open reading frame (ORF) of 1146 and 891bp respectively and showed high similarity to other plant genes. AhITPK1 shared high homology with Aradu. Q95MC of Arachis duranensis, had a single exon with no introns and belonged to ATP-grasp family of proteins. AhIPK2 shared high similarity with Aradu.24V9G of A. duranensis and contained three exons with 5’ and 3’ UTR’s on either side. Unlike other IPK2 genes, AhIPK2 possessed conserved domains such as PxxxDxKxG and [L/M][I/V]D[F/L][A/G][H/K]. Phylogenetic analysis grouped AhITPK1 with A. duranensis, A. ipinensis and Oryza brachyantha into one cluster, whereas AhIPK2 was grouped along with Cucumis melo and C. sativus. Evolutionarily, AhITPK1 and AhIPK2 were genetically distinct from other plant genera. Furthermore, real-time PCR analysis revealed high expression of AhITPK1 and AhIPK2 genes in the peanut embryo and flower bud. For the first time AhITPK1 (KR778986) and AhIPK2 (KR778988) genes belonging to phytic acid pathway from Arachis hypogaea were identified and characterized the expression pattern of these two isoforms on different tissues. These genes were found to be abundant in flower bud and embryo. Results suggest that embryo development significantly influences the expression of the two AhIPK isoforms in peanut. Evolutionarily they were found to be distinct from their parental species. This study is an important step toward understanding the role of these two AhIPK isoforms in phytic acid synthesis. However, future research involving RNAi-based functional characterization is warranted to establish their link to embryo development in peanut. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | United States Department of Agriculture (USDA) under Norman E. Borlaug International Agricultural Science and Technology Fellowship Program (Borlaug Fellowship Program) 2014 |
Language: | English |
Name of Journal: | Cloning and Transgenics |
Volume No.: | 5 |
Page Number: | Not Available |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | dx.doi.org/10.4172/2168-9849.1000150 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/33412 |
Appears in Collections: | CS-DGR-Publication |
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