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http://krishi.icar.gov.in/jspui/handle/123456789/34798
Title: | Optimising protocol for successful development of haploids in marigold (Tagetes spp.) through in vitro androgenesis |
Other Titles: | Not Available |
Authors: | K. Ravindra Kumar · Kanwar Pal Singh · Reeta Bhatia · D. V. S. Raju · Sapna Panwar |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Indian Agricultural Research Institute ICAR::Directorate of Floricultural Research |
Published/ Complete Date: | 2019-03-30 |
Project Code: | Not Available |
Keywords: | African marigold · French marigold · Anther culture · Haploid · Flow cytometry · Cytology · Chloroplast |
Publisher: | Not Available |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | For the frst time, we have developed a protocol for rapid and successful induction of haploids in Tagetes spp. using in vitro androgenesis techniques. Various factors afecting the in vitro androgenesis response in Tagetes spp. were optimised. At frst, a correlation was established between foret size/bud size and stage of microspore development in diferent genotypes of Tagetes erecta and Tagetes patula. The foret size of 3.5–4.0 mm (French marigold) and 3.0–3.5 mm (African marigold) contained the highest percent of microspores at early-uninucleate to early-binucleate stage. The frequency of shoot formation from anthers ranged from 0 to 78.6% in T. patula cv. Pusa Arpita and 0 to 63.9% in T. erecta cv. Pusa Basanti Gainda. The French marigold cultivars exhibited higher androgenic response over the African marigold cultivars. The efects of basal media, plant growth regulator combinations and sucrose concentration for direct diferentiation of shoot buds and haploid induction were studied. Marigold anthers cultured on newly formulated EMS1 basal media (enriched with coconut water, AgNO3, PVP etc.) exhibited a very high androgenic response over the other commercially available media. We have also optimised the cold temperature pre-treatments and culture condition for high frequency androgenesis in marigold. Ten days cold pre-treated anthers along with 20 days dark incubation of cultures proved highly benefcial for haploid induction. Among a total of 424 anther-regenerants, 56 plants were randomly selected for fow cytometry and cytological analysis. The ploidy analysis revealed 14.3% of anther regenerants as dihaploids, 66.1% as tetraploid (similar to donor mother plant) and 19.6% as polyploid. The determination of ploidy level by counting the number of chloroplast in stomatal guard cells of marigold was also established for rapid screening of haploids. The identifed haploids were successfully diploidised and are being utilised in the hybrid breeding programme at our institute. The developed protocol will facilitate doubled haploids based breeding in Tagetes spp. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Plant Cell Tissue and Organ Culture |
NAAS Rating: | 8.2 |
Page Number: | Not Available |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | https://doi.org/10.1007/s11240-019-01598-3 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/34798 |
Appears in Collections: | HS-DFR-Publication |
Files in This Item:
File | Description | Size | Format | |
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Optimising protocol for successful development of haploidsKumar2019.pdf | 1.94 MB | Adobe PDF | View/Open |
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