KRISHI
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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/41959
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Mohanty S | en_US |
dc.contributor.author | M. Makesh | en_US |
dc.contributor.author | Rajendran K.V | en_US |
dc.contributor.author | Babu S | en_US |
dc.contributor.author | Anand D | en_US |
dc.contributor.author | Sujeet Kumar | en_US |
dc.contributor.author | Kumar A | en_US |
dc.contributor.author | Baitha R | en_US |
dc.contributor.author | Sarma K | en_US |
dc.date.accessioned | 2020-10-17T07:03:29Z | - |
dc.date.available | 2020-10-17T07:03:29Z | - |
dc.date.issued | 2020-06-01 | - |
dc.identifier.citation | Not Available | en_US |
dc.identifier.issn | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/41959 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Serum immunoglobulins (Ig) of mrigal Cirrhinus mrigala (Hamilton 1822) immunised with bovine serum albumin (BSA), were purified by affinity chromatography using BSA-CL agarose column. The purified mrigal Ig (m-Ig) was characterised under reducing condition by Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) which revealed two bands of 85 and 26 kDa corresponding to heavy and light chain, respectively. Following fusion of splenocytes from Balb/c mice immunised with purified m-Ig with myeloma cells, three hybridomas showing reactivity with m-Ig were cloned by limiting dilution. The monoclonal antibodies (MAbs) generated by these clones were designated as 3B2-E12, 3B2-F9 and 4C3-B2 and characterised by western blotting and isotyping. Western blot analysis of the supernatant from the three clones with purified m-Ig indicated that, all the three MAbs were specific to heavy chain. Isotyping revealed that 3B2-E12 MAb was of IgG1 isotype whereas the other two MAbs were of IgG2a isotype. Cross reactivity of anti-mrigal Ig MAb (3B2-E12) was observed with serum Ig of Catla catla and Labeo rohita indicating semi-conserved nature of Ig in Indian major carps. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Not Available | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Cirrhinus mrigala | en_US |
dc.subject | ELISA | en_US |
dc.subject | Immunoglobulin | en_US |
dc.subject | Monoclonal Antibodies and | en_US |
dc.subject | Western blotting | en_US |
dc.title | Production and characterisation of monoclonal antibodies against immunoglobulins of Cirrhinus mrigala (Hamilton 1822) | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Article | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Indian Journal of Fisheires | en_US |
dc.publication.volumeno | Not Available | en_US |
dc.publication.pagenumber | Not Available | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | 10.21077/ijf.2019.67.2.84594-08 | en_US |
dc.publication.authorAffiliation | ICAR::Central Institute of Brackishwater Aquaculture | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.naasrating | 6.29 | en_US |
Appears in Collections: | FS-CIBA-Publication |
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