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http://krishi.icar.gov.in/jspui/handle/123456789/43590
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sharma R | en_US |
dc.contributor.author | H.R. Mahla | en_US |
dc.contributor.author | T. Mohapatra | en_US |
dc.contributor.author | S.C. Bhargava | en_US |
dc.date.accessioned | 2020-12-23T10:49:28Z | - |
dc.date.available | 2020-12-23T10:49:28Z | - |
dc.date.issued | 2003-03-01 | - |
dc.identifier.citation | Sharma R, H.R. Mahla, T. Mohapatra and S.C. Bhargava (2003). Isolating Plant Genomic DNA without Liquid Nitrogen. Plant Molecular Biology Reporter 21(1): 43-50. | en_US |
dc.identifier.issn | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/43590 | - |
dc.description | Not Available | en_US |
dc.description.abstract | DNA was isolated from leaves of 10 plant species (cuminum cyminum, vigna aconitifolia, pennisetum typhoides, Tecoma stans, Lycium barbarum, Anogeissus acuminate, Tecomella undulata, zizphus mauritiana, Phoenix dactylifera, and Erucasativa ) and a fungus (Fusarium oxysporum ) using the CTAB method. Three fixing solutions (alcohol, alcohol and chloroform , alcohol and EDTA ) were used to produce high molecular weight dna (>40 kb). DNA quality was comparable for the 3 fixing solution offered no advantage over absolute alcohol. Isolated DNA was suitable for RAPD analysis, restriction digestion , abd cloning. This method does not require liquid nitrogen for fixation , grinfing, or storage at- 80 c, making it advantageous over other common protocols. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Not Available | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | cloning | en_US |
dc.subject | CTAB | en_US |
dc.subject | DNA isolation | en_US |
dc.subject | genomic DNA | en_US |
dc.subject | RAPD | en_US |
dc.subject | restriction | en_US |
dc.title | Isolating Plant Genomic DNA without Liquid Nitrogen | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Plant Molecular Biology | en_US |
dc.publication.volumeno | 21(1) | en_US |
dc.publication.pagenumber | 43-50 | en_US |
dc.publication.divisionUnit | Div.III (PI&PM) | en_US |
dc.publication.sourceUrl | https://link.springer.com/article/10.1007/BF02773395 | en_US |
dc.publication.authorAffiliation | ICAR-CAZRI, Jodhpur | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.naasrating | 9.3 | en_US |
Appears in Collections: | NRM-CAZRI-Publication |
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