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http://krishi.icar.gov.in/jspui/handle/123456789/44852
Title: | An efficient in-vitro Agrobacterium -mediated transformation protocol for raising salinity tolerant transgenic plants in finger millet [Eleusine coracana (L.) Gaertn.] |
Authors: | B.G. Jayasudha, A.M. Sushma, Prashantkumar S Hanjagi and V.R. Sashidhar. |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | Department of Crop Physiology, University of Agricultural Sciences, Bengaluru and ICAR-National Rice Research Institute, Cuttack ICAR::National Rice Research Institute |
Published/ Complete Date: | 2014-10-01 |
Keywords: | Fingermillet, Transgenics, Transformation, Salt tolerance |
Publisher: | Plant Archives |
Citation: | Jayasudha BG, Sushma MA, Prashantkumar SH and Sashidhar VR (2014). An efficient in-vitro Agrobacterium -mediated transformation protocol for raising salinity tolerant transgenic plants in finger millet [Eleusine coracana (L.) Gaertn.]. Plant Archives Vol. 14 No. 2, 2014 pp. 823-829 |
Series/Report no.: | Not Available; |
Abstract/Description: | An efficient reproducible protocol has been established for raising salinity tolerant fingermillet through in vitro Agrobacterium mediated transformation. To enhance the salinity tolerance in fingermillet a double gene construct of PgNHX1 (from Pennisetum glaucum) and AVP1 (from Arabidopsis thaliana) was developed using the plant binary expression vector pCAMBIA 1301. This was then mobilized into electro competent grobacterium tumefaciens strain EHA105 and used for transforming fingermillet var. GPU28. GUS histochemical assay was monitored for confirmation of callus transformation. The putative transformants were acclimatized in incubation chamber for two weeks and then transplanted in greenhouse for further acclimatization. Putative transgenics were confirmed by physiological analysis and PCR amplification of genomic DNA using primers. The putative transgenic plants showed higher salt tolerance of 300mM compared to treated wild type plants. This is the first study in fingermillet reporting the expression of double gene construct of PgNHX1 and AVP1 for salinity tolerance. |
Description: | Not Available |
ISSN: | 0972-5210 |
Type(s) of content: | Research Paper |
Sponsors: | DST (Department of Science and Technology), New Delhi, India. |
Language: | English |
Name of Journal: | Plant Archives |
NAAS Rating: | Not Available |
Volume No.: | 14 (2) |
Page Number: | 823-829 |
Name of the Division/Regional Station: | Crop Physiology and Biochemistry Division |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/44852 |
Appears in Collections: | CS-NRRI-Publication |
Files in This Item:
File | Description | Size | Format | |
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Jayasudha et al_2014_Finger millet_Protocol Paper.pdf | 1.12 MB | Adobe PDF | View/Open |
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