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http://krishi.icar.gov.in/jspui/handle/123456789/46567
Title: | Comparative evaluation of fluorescence polarization assay and competitive ELISA for the diagnosis of bovine brucellosis vis-a-vis sero-monitoring |
Other Titles: | Not Available |
Authors: | Kalleshamurthy T Skariah S Rathore Y Kavana DR Nagaraj C Shome BR Rahman H Barman NN Shome R |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR: National Institute of Veterinary Epidemiology and Disease Informatics Jain University, Centre for Post Graduate Studies, Jayanagar, Bengaluru International Livestock Research Institute (ILRI), Block-C, First Floor, NASC Complex, CG Centre, DPS Marg, Pusa, New Delhi Department of Microbiology, College of Veterinary Sciences, Khanapara, Guwahati, Assam |
Published/ Complete Date: | 2020-01-31 |
Project Code: | Not Available |
Keywords: | ELISA FPA iELISA RBPT Seroprevalence |
Publisher: | Elsevier Publishers private limited |
Citation: | Kalleshamurthy T, Skariah S, Rathore Y, Kavana DR, Nagaraj C, Shome BR, Rahman H, Barman NN and Shome R. (2020). Comparative evaluation of fluorescence polarization assay and competitive ELISA for the diagnosis of bovine brucellosis vis-a-vis sero-monitoring. Journal of Microbiological Methods. 170: 1-6 |
Series/Report no.: | Not Available; |
Abstract/Description: | Brucellosis is an important zoonosis that constitutes a serious public health hazard which is caused by a bacterium belonging to the genus Brucella. In the present study, two highly specific serological tests for brucellosis diagnosis, fluorescence polarization assay (FPA) and competitive ELISA (cELISA) were standardized in the laboratory, evaluated and compared with rose bengal plate test (RBPT), indirect ELISA (iELISA) and commercial cELISA kit. For test evaluation, 1386 serum samples [apparently healthy animals (n = 260), samples from Brucella infected farms (n = 701) and B. abortus S19 vaccinated animals (n = 425)] were analyzed to assess suitable diagnostic test in B. abortus S19 post vaccinated bovine population. In apparently healthy brucellosis free farms, RBPT, iELISA, in-house FPA and cELISA were found to be highly specific than commercial cELISA. Commercial cELISA kit was comparatively more sensitive than other serological tests in samples collected from infected farms. The FPA showed sensitivity nearly equal to RBPT and in-house cELISA showed greater sensitivity than RBPT in infected farms. In animals with persistent vaccinal antibodies, only in-house FPA and cELISA recorded higher specificity of 87.64 and 90.27%, respectively. The other tests, RBPT and iELISA displayed similar reactivity with vaccine antibodies to that of infection antibodies whereas commercial cELISA kit showed an intermediate specificity of 47.69%. With these findings, RBPT, iELISA and cELISA are suggested for screening infected herds, and in-house developed FPA and cELISA tests with a proven specificity can be used for confirmatory diagnosis of brucellosis in B. abortus S19 post vaccinated animal populations. |
Description: | Not Available |
ISSN: | 0167-7012 |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Journal of Microbiological Methods |
Journal Type: | International Journal |
NAAS Rating: | 7.71 |
Impact Factor: | 1.707 |
Volume No.: | 170 |
Page Number: | 1-6 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | https://doi.org/10.1016/j.mimet.2020.105858 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/46567 |
Appears in Collections: | AS-NIVEDI-Publication |
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