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http://krishi.icar.gov.in/jspui/handle/123456789/4798
Title: | Development of EST derived SSRs and SNPs as a genomic resource in Indian catfish, Clarias batrachus |
Other Titles: | Not Available |
Authors: | Vindhya Mohindra Akanksha Singh A. S. Barman Ratnesh Tripathi Neeraj Sood Kuldeep K. Lal |
Published/ Complete Date: | 2012-05 |
Project Code: | Not Available |
Keywords: | Indian catfish Clarias batrachus Spleen cDNA library Expressed sequence tags Type I microsatellite Single nucleotide polymorphism |
Publisher: | SPRINGER |
Citation: | Mohindra, V., Singh, A., Barman, A.S., Tripathi, R., Sood, N. and Lal, K.K., 2012. Development of EST derived SSRs and SNPs as a genomic resource in Indian catfish, Clarias batrachus. Molecular biology reports, 39(5), pp.5921-5931. |
Series/Report no.: | Not Available; |
Abstract/Description: | Clarias batrachus, an Indian catfish species, is endemic to the Indian subcontinent and potential cultivable species. The genomic resources in C. batrachus in the form of ESTs containing microsatellite repeats (EST-SSR) and single nucleotide polymorphisms (SNPs) that are associated with the expressed genes from spleen were mined. From a total of 1,937 ESTs generated, 1,698 unique sequences were obtained, out of which 221 EST-SSRs were identified and 54% could be functionally annotated by similarity searches. A total of 23 contigs containing 3 or more ESTs were found to contain 31 SNP loci, out of which 8 ESTs showed similarity to genes of known function and 1 for hypothetical protein. Nine ESTs with SSRs and/or SNPs identified in this study were reported to be associated with diseases in human and animals. These identified loci can be developed into markers in C. batrachus, which can be useful in linkage mapping, comparative genomics studies and for its genetic improvement programmes. |
Description: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Department of Biotechnology, Government of India |
Language: | English |
Name of Journal: | Molecular Biology Reports |
NAAS Rating: | 7.4 |
Volume No.: | 39(5) |
Page Number: | 5921-5931 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | 10.1007/s11033-011-1404-z |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/4798 |
Appears in Collections: | FS-NBFGR-Publication |
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art%3A10.1007%2Fs11033-011-1404-z.pdf | 499.53 kB | Adobe PDF | View/Open |
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