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http://krishi.icar.gov.in/jspui/handle/123456789/50634
Title: | Designing of siRNAs for various target genes of Cucumber mosaic virus subgroup IB. |
Other Titles: | Not Available |
Authors: | K.A. Revathy A. I. Bhat |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR-Indian Institute of Spices Research |
Published/ Complete Date: | 2019-03-18 |
Project Code: | SR/WOS-A/LS-1429/2015 |
Keywords: | Cucumber mosaic virus, black pepper, RNA interference, siRNA designing, hairpin construct |
Publisher: | CSIR-NISCAIR |
Citation: | Revathy K A, Bhat , A.I. 2019. Designing of siRNAs for various target genes of Cucumber mosaic virus subgroup IB. Indian J. Biotech. 18: 119-125. |
Series/Report no.: | Not Available; |
Abstract/Description: | Cucumber mosaic virus (CMV) is a major production constraint in black pepper causing stunted disease. Resistant varieties are unavailable and control measures are not effective till now. Recently, RNA interference is the most promising strategy for combating virus infection in plants but the effectiveness depends on sequence specificity between the transgene and the targeting virus. This study was undertaken to design the most suitable region for double stranded RNA synthesis with maximum specificity and minimized off–targets for all the five genes of CMV from black pepper. A 400 bp off–target minimized region identified from each of the five genes was subjected to sequence polymorphism study with selected CMV subgroup IB strains and common siRNAs were designed in silico. As 3b gene had the least variations (of 17%) with four common and potential siRNAs designed from this region in silico, a hairpin construct was assembled using this region in Agrobacterium that can be used for developing black pepper resistant to selected CMV subgroup IB strains |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Indian Journal of Biotechnology |
Journal Type: | Research Journal |
NAAS Rating: | 6.41 |
Impact Factor: | 0.414 |
Volume No.: | 18 |
Page Number: | 119-125 |
Name of the Division/Regional Station: | Crop Protection |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/50634 |
Appears in Collections: | HS-IISR-Publication |
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