KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/50893
Title: | Identification and characterization of catalase genes in Eleusine coracana under abiotic stresses. |
Other Titles: | Not Available |
Authors: | Singh S, Ramakrishna Ch, Reddy N, Khan S, Padaria JC and Solanke AU |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR - National Institute for Plant Biotechnology, Pusa Campus, New Delhi 110012, India Banasthali University, Banasthali, Rajasthan 304022, India All India Coordinated Research Project on Small Millets, ICAR, GKVK, Bangalore 560065, India |
Published/ Complete Date: | 2019-02-06 |
Project Code: | Not Available |
Keywords: | finger millet, gene expression and cloning, heat, mannitol, reactive oxygen species, salinity |
Publisher: | Not Available |
Citation: | Singh S, Ramakrishna Ch, Reddy N, Khan S, Padaria JC and Solanke AU (2019) Identification and characterization of catalase genes in Eleusine coracana under abiotic stresses. Biologia Plantarum, 63: 440-447 (DOI: 10.32615/bp.2019.048) |
Series/Report no.: | Not Available; |
Abstract/Description: | Reactive oxygen species (ROS) are byproducts of metabolic processes such as respiration and photosynthesis in plants. Production of ROS leads to rapid cell damage, and plants developed a complex system of enzymatic and non-enzymatic antioxidants to scavenge these ROS. Catalase is an important enzyme, which plays a key role in elimination of toxic effects of hydrogen peroxide and plays a major role as an antioxidant. When characterizing heat responsive genes in finger millet (Eleusine coracana L.) using a suppression subtractive hybridization (SSH) library, we isolated two catalase genes and named them as EcCATA1 and EcCATB1. The lengths of the EcCATA1 and EcCATB1 open reading frames were 1 482 and 1 426 bp, respectively. We characterized these genes under different abiotic stresses and in different tissues. The tissue wise expression revealed that EcCATA1 expression was higher in leaves whereas EcCATB1 expression was higher in roots than in other organs. Under stress conditions, the expression of EcCATA1 was highest under salt stress followed by mannitol treatment. In the case of EcCATB1, the highest expression was observed under mannitol treatment followed by cold and dehydration. We also studied expression of both the genes under heat stress in different finger millet genotypes and observed that expressions of these genes can be correlated with heat tolerance. For both the genes, a detailed computational investigation was also performed for understanding their structural properties and physicochemical characteristics. Overall, this is the first study to identify and characterize catalase genes from climate resilient finger millet crop. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Biologia Plantarum |
NAAS Rating: | 7.6 |
Impact Factor: | 1.56 |
Volume No.: | Not Available |
Page Number: | Not Available |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | DOI: 10.32615/bp.2019.048 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/50893 |
Appears in Collections: | CS-NIPB-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.