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http://krishi.icar.gov.in/jspui/handle/123456789/53498
Title: | Development of a real-time PCR based protocol for quantifying Radopholus similis in field samples. |
Other Titles: | Not Available |
Authors: | P B Krishna; S J Eapen |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Indian Institute of Spices Research |
Published/ Complete Date: | 2019-08-02 |
Project Code: | Not Available |
Keywords: | burrowing nematode, diagnostics, ITS, Radopholus similis, Real-Time PCR, SYBR green |
Publisher: | Indian Society for Spices |
Citation: | P B Krishna, S J Eapen (2019). Development of a Real-Time PCR based protocol for quantifying Radopholus similis in field samples. Journal of Spices and Aromatic Crops, 28(1), 52–60. https://doi.org/10.25081/josac.2019.v28.i1.5744 |
Series/Report no.: | Not Available; |
Abstract/Description: | The burrowing nematode, Radopholus similis, is an obligate migratory endoparasite. Currently detection of this nematode is carried out mostly by physically extracting them from soil and then observing under a light microscope. To identify this nematode, a thorough knowledge about their morphological features is quite indispensable. Developing a DNA based detection technique makes it more convenient and accurate in detection. Though PCR based methods have been reported by earlier workers, developing a Real-Time PCR based method will enable estimating their population in field samples. In this study, Real-Time PCR primers were designed using the DNA sequences from the ITS region of R. similis. It can detect R. similis up to the limit of 100 fg μL-1 DNA. The real time PCR based detection serves as an efficient tool for the detection and estimation of this nematode from soil samples. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Journal of Spices and Aromatic Crops |
Volume No.: | 28(1) |
Page Number: | 52-60 |
Name of the Division/Regional Station: | Division of Crop Protection |
Source, DOI or any other URL: | https://doi.org/10.25081/josac.2019.v28.i1.5744 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/53498 |
Appears in Collections: | HS-IISR-Publication |
Files in This Item:
File | Description | Size | Format | |
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editor-journal-editor-josac-vol.-28-1-52-60-krishna--eapen.pdf | 350.45 kB | Adobe PDF | View/Open |
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