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http://krishi.icar.gov.in/jspui/handle/123456789/57237
Title: | Rapid polymerase chain reaction (PCR) for detection of pathogenic leptospires in biological samples |
Other Titles: | Not Available |
Authors: | Bhure SK Lakshmisha KV Patil SS Shome BR Shome R Gangadhar NL Gajendragad MR Prabhudas K |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | Project Directorate on Animal Disease Monitoring and Surveillance, Bengaluru |
Published/ Complete Date: | 2009-08-10 |
Project Code: | Not Available |
Keywords: | Leptospirosis Lip L32 gene Rapid PCR |
Publisher: | Indian Journal of Animal Sciences |
Citation: | Bhure SK, Lakshmisha KV, Patil SS, Shome BR, Shome R, Gangadhar NL, Gajendragad MR and Prabhudas K. 2009.Rapid polymerase chain reaction (PCR) for detection of pathogenic leptospires in biological samples.Indian Journal of Animal Sciences 79(8): 781–783 |
Series/Report no.: | Not Available; |
Abstract/Description: | Leptospirosis, considered to be the most widespread zoonoses in the world, is caused by serovars belonging to 7 pathogenic species of the genus Leptospira, viz. L. borgpetersenii, L. inadai, L. interrogans, L. kirschneri, L. noguchii, L. santarosai, and L. weilii (Ramadass et al. 1992). This paper reports the development of molecular technique for detecion of pathogenic Leptospira species by PCR amplification of a partial fragment of the major outer- membrane lipoprotein LipL32 gene, whose protein appears to be an important virulence factor (Haake et al. 2000, Yang et al. 2002), confined to pathogenic strains of all Leptospira spp. The technique could detect majority of serovars of L. interrogans, viz. icterohaemorrhagiae, canicola, autumnalis, javonica, pomona, pyrogenes, australis; L. borgpetersenii serovar Hardjobovis, and L. inadai. |
Description: | Not Available |
ISSN: | 0367-8318 |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Indian Journal of Animal Sciences |
Journal Type: | National Journal |
NAAS Rating: | 6.28 |
Impact Factor: | 0.28 |
Volume No.: | 79(8) |
Page Number: | 781-783 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/3087/1154 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/57237 |
Appears in Collections: | AS-NIVEDI-Publication |
Files in This Item:
File | Description | Size | Format | |
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Bhure et al 2009.pdf | 51.97 kB | Adobe PDF | View/Open |
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