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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/57602
Title: | High Frequency Induction of Multiple Shoots and Plant Regeneration from Cotyledonary Nodal Explant of Mung Bean [Vigna radiata (L) Wilczek] |
Other Titles: | Not Available |
Authors: | S. K. Yadav Maheswari Mandapaka Vanaja M |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR-CRIDA |
Published/ Complete Date: | 2010-07-01 |
Project Code: | Not Available |
Keywords: | BAP half cotyledonary node with intact cotyledon mung bean regeneration |
Publisher: | Not Available |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | An efficient protocol for shoot bud induction and proliferation employing half cotyledonary node with intact cotyledon explants derived from two-day-old seedlings of mung bean pre-conditioned on 6- benzylaminopurine (BAP) has been achieved. Explants were cultured for four weeks each on MS B5 + 12.5 μM BAP and MS B5 + 5 μM BAP +0.05 μM α- naphthaleneacetic acid (NAA ), respectively, as shoot bud induction and shoot elongation and proliferation media, gave the best regeneration response. The removal of the pre-existing buds from explants at 12 days in shoot bud induction medium led to enhanced regeneration response. Light microscopic observations on 14-day-old explants confirmed direct organogenesis route of regeneration. Elongated shoots (>2 cm) excised from the regenerating cultures were successfully rooted on half MS B5 medium containing 2.46 μM indolebutyric acid (IBA). About 90% of the rooted plantlets, efficiently hardened in pots having soil and farm yard manure, flowered and produced pods with viable seeds upon reaching maturity. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Journal of Plant Biochemistry and Biotechnology |
NAAS Rating: | 6.77 |
Volume No.: | Not Available |
Page Number: | 267-270 |
Name of the Division/Regional Station: | DCS |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/57602 |
Appears in Collections: | NRM-CRIDA-Publication |
Files in This Item:
File | Description | Size | Format | |
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JPBB 2010 SKY (1).pdf | 146.8 kB | Adobe PDF | View/Open |
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