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http://krishi.icar.gov.in/jspui/handle/123456789/60760
Title: | Rapid screening test for detection of oxytetracycline residues in milk using lateral flow assay |
Other Titles: | Not Available |
Authors: | Naik, L., Sharma, R., Mann, B., Lata, K., Rajput, Y.S. and Nath, B.S., |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::National Dairy Research Institute |
Published/ Complete Date: | 1001-01-01 |
Project Code: | Not Available |
Keywords: | Milk Oxytetracycline Lateral flow assay Gold nanoparticles Antibiotics |
Publisher: | Not Available |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | A rapid, semi-quantitative lateral flow assay (LFA) was developed to screen the oxytetracycline (OTC) antibiotics residues in milk samples. In this study a competitive immuno-assay format was established. Colloidal gold nano-particles (GNP) were prepared and used as labelling material in LFA. Polyclonal anti bodies were generated against OTC molecule (anti-OTC), purified and the quality was assessed by enzyme linked immuno sorbet assay. For the first time membrane components required for LFA in milk system was optimized. GNP and anti-OTC stable conjugate preparation method was standardized, and then these components were placed over the conjugate pad. OTC coupled with carrier protein was placed on test line; species specific secondary antibodies were placed on the control line of the membrane matrix. Assay was validated by spiking OTC to antibiotic free milk samples and results could be accomplished within 5 min. without need of any equipment. The visual detection limit was 30 ppb. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Not Available |
Volume No.: | Not Available |
Page Number: | Not Available |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/60760 |
Appears in Collections: | AS-NDRI-Publication |
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