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Title: | Genome-wide identification and analysis of biotic and abiotic stress regulation of small heat shock protein (HSP20) family genes in bread wheat. |
Other Titles: | Not Available |
Authors: | Muthusamy SK, Dalal M, Chinnusamy V, Bansal KC |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Indian Agricultural Research Institute ICAR::National Research Centre on Plant Biotechnology ICAR::Indian Institute of Wheat and Barley Research |
Published/ Complete Date: | 2017-01-22 |
Project Code: | Not Available |
Keywords: | sHSPTaHSP20 -Crystalline C-terminal domainTriticum aestivumHigh temperatureColdSalt stressAbiotic |
Publisher: | Elsevier |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | tSmall Heat Shock Proteins (sHSPs)/HSP20 are molecular chaperones that protect plants by preventingprotein aggregation during abiotic stress conditions, especially heat stress. Due to global climate change,high temperature is emerging as a major threat to wheat productivity. Thus, the identification of HSP20and analysis of HSP transcriptional regulation under different abiotic stresses in wheat would help inunderstanding the role of these proteins in abiotic stress tolerance. We used sequences of known riceand Arabidopsis HSP20 HMM profiles as queries against publicly available wheat genome and wheat fulllength cDNA databases (TriFLDB) to identify the respective orthologues from wheat. 163 TaHSP20 (includ-ing 109 sHSP and 54 ACD) genes were identified and classified according to the sub-cellular localizationand phylogenetic relationship with sequenced grass genomes (Oryza sativa, Sorghum bicolor, Zea mays,Brachypodium distachyon and Setaria italica). Spatio-temporal, biotic and abiotic stress-specific expres-sion patterns in normalized RNA seq and wheat array datasets revealed constitutive as well as inductiveresponses of HSP20 in different tissues and developmental stages of wheat. Promoter analysis of TaHSP20genes showed the presence of tissue-specific, biotic, abiotic, light-responsive, circadian and cell cycle-responsive cis-regulatory elements. 14 TaHSP20 family genes were under the regulation of 8 TamiRNAgenes. The expression levels of twelve HSP20 genes were studied under abiotic stress conditions in thedrought- and heat-tolerant wheat genotype C306. Of the 13 TaHSP20 genes, TaHSP16.9H-CI showed highconstitutive expression with upregulation only under salt stress. Both heat and salt stresses upregulatedthe expression of TaHSP17.4-CI, TaHSP17.7A-CI, TaHSP19.1-CIII, TaACD20.0B-CII and TaACD20.6C-CIV, whileTaHSP23.7-MTI was specifically induced only under heat stress. Our results showed that the identifiedTaHSP20 genes play an important role under different abiotic stress conditions. Thus, the results illustratethe complexity of the TaHSP20 gene family and its stress regulation in wheat, and suggest that sHSPs asattractive breeding targets for improvement of the heat tolerance of wheat. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Journal of Plant Physiology |
Volume No.: | 211 |
Page Number: | 100-113 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | https://www.ncbi.nlm.nih.gov/pubmed/28178571 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/6204 |
Appears in Collections: | CS-NIPB-Publication |
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