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http://krishi.icar.gov.in/jspui/handle/123456789/67055
Title: | Evaluation of interferon gamma (IFN-?) of Labeo rohita as an immunomodulator: in vitro expression model. |
Authors: | Megha kadam Bedikar Praveena Soman Sajal Kole Deepika Anand Gayatri Tripathi M. Makesh K.V.Rajendran |
Published/ Complete Date: | 2018-8-10 |
Keywords: | IFN-?, Labeo rohita, Peripheral blood mononuclear cells, Poly I:C |
Publisher: | Not Available |
Citation: | Bedekar, M.K., Soman, P., Kole, S., Anand, D., Tripathi, G., Makesh, M. and Rajendran, K.V., 2018. Evaluation of interferon gamma (IFN-?) of Labeo rohita as an immunomodulator: in vitro expression model. Aquaculture international. Vol. & Issue: 26(6), pp.1401-1413 |
Abstract/Description: | Interferon gamma (IFN-?) or type II interferon is a cytokine that is critical for innate and adaptive immunity against viral and some bacterial and protozoal infections. The importance of IFN-? in the immune system lies in its ability to inhibit viral replication directly and most importantly from its immunomodulatory effects. Previously, we successfully co-administered IFN-? along with GAPDH gene of Edwardsiella tarda as bicistronic DNA vaccine in Labeo rohita. In order to ascertain the individual role of IFN-?, the present study involves cloning and expression of 552-bp IFN-? open-reading frame (ORF) of L. rohita in striped snakehead (SSN-1) cell line using eukaryotic expression vector system (pQE-TriSystem) followed by transfection in peripheral blood lymphocytes (PBMCs) to evaluate its immunomodulatory ability in comparison to polyinosinic-polycytidylic acid (Poly I:C)-treated PBMCs. The 18.7-kDa protein, expressed in the pQE-IFN?-transfected SSN-1 cells, reacted with anti-His antibody in Western blot confirming it to be recombinant IFN-?, whereas the relative expression of IFN-?, iNOS, Mx, and IL-1? genes in PBMCs was quantified at 24 h and 48 h post treatment by qPCR. The comparative kinetics of all four genes showed significantly (p?<?0.05) high upregulation pattern in both pQE-IFN?-transfected cell group and Poly I:C-treated cell group demonstrating recombinant IFN-? as an equally efficient inducer like Poly I:C. Thus, our in vitro experiment results highlight the immunomodulatory potential of recombinant IFN-? as an analogue to synthetic Poly I:C which warranted future studies to further explore the potential of recombinant IFN-? as an effective vaccine adjuvant against different microbial invasion. |
Description: | Not Available |
ISBN: | Not Available |
ISSN: | 1573-143X |
Type(s) of content: | Research Paper |
Language: | English |
Name of Journal: | Aquaculture international |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/67055 |
Appears in Collections: | FS-CIFE-Publication |
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