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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/72879
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ahmed I, Bhat I, Jagtap A, Balasinor D, Kumar NS, Chanu TI, Rani B, Dar S, Leya T, Gora A, Saharan N* | en_US |
dc.date.accessioned | 2022-06-18T05:49:29Z | - |
dc.date.available | 2022-06-18T05:49:29Z | - |
dc.date.issued | 2018-11-10 | - |
dc.identifier.citation | Not Available | en_US |
dc.identifier.issn | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/72879 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Inhibins are heterodimeric glycoproteins that belong to transforming growth factor-β superfamily. The present study was aimed at structural elucidation of immunogenic inhibin chimeric peptide (ICP) using immunoinfor- matics tools, to develop anti-ICP antibodies and to understand the mechanism of interaction between HPG-axis and anti-ICP antibodies in adult female Clarias batrachus. Homology modelling predicted that the ICP adopts a turn and random coil, which was further confirmed by circular dichroism (CD). Competitive binding ELISA with ICP and different unrelated peptides confirmed that the developed anti-ICP antiserum is highly specific and critical for the direct interaction to the inhibin-α subunit. The anti-ICP antibodies increased the mRNA transcript levels of follicle stimulating hormone receptor (FSHr), luteinizing hormone receptor (LHr), activin receptor type I (Act RI) and activin receptor type II (Act RII). Further, the serum concentrations of follicle stimulating hormone (FSH), 17β-estradiol (E2) and progesterone increased significantly in anti-ICP antisera treated groups compared to control. Modelled inhibin-α and ICP exhibits better interaction with each other through two salt bridge formations. Also, ICP showed better interaction with betaglycan through positive and polar-based interactions. The results of the present study suggest that immunoneutralization of inhibin bioactivity, confirms the phy- siological role of inhibin as a component of ovarian feedback mechanism regulating FSH secretion through augmentation of gonadotropin and activin receptor signalling pathways that could affect the reproductive success in fishes. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | ELSEVIER | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Inhibin chimeric peptide Circular dichroism Protein-peptide docking Anti-peptide antibody Activin receptor signalling | en_US |
dc.title | Computational and biological approach for studying structure-function of inhibin chimeric peptide antibodies in Clarias batrachus. | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Aquaculture | en_US |
dc.publication.volumeno | 501 | en_US |
dc.publication.pagenumber | 153-60 | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | Not Available | en_US |
dc.publication.authorAffiliation | ICAR: Central Institute of Fisheries Education | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.journaltype | International | en_US |
dc.publication.naasrating | 8.71 | en_US |
dc.publication.impactfactor | 4.242 | en_US |
Appears in Collections: | FS-CIFE-Publication |
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