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http://krishi.icar.gov.in/jspui/handle/123456789/74560
Title: | Androgen receptor gene deficiency results in the reduction of steroidogenic potential in porcine luteal cells |
Other Titles: | Not Available |
Authors: | Not AvailableJaya Bharati, Satish Kumar, Sunil Kumar, NH Mohan, Rafiqul Islam, Seema Rani Pegu, Santanu Banik, Bikash Chandra Das, Sanjib Borah, Mihir Sarkar |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::National Research Centre on Pig |
Published/ Complete Date: | 2022-06-08 |
Project Code: | IXX15063 |
Keywords: | Androgen receptor; steroidogenesis; proliferation; viability; luteal cell; porcine |
Publisher: | Taylor and Francis |
Citation: | Bharati, Jaya, Satish Kumar, Sunil Kumar, N. H. Mohan, Rafiqul Islam, Seema Rani Pegu, Santanu Banik, Bikash Chandra Das, Sanjib Borah, and Mihir Sarkar. "Androgen receptor gene deficiency results in the reduction of steroidogenic potential in porcine luteal cells." Animal Biotechnology (2022): 1-14. |
Series/Report no.: | Not Available; |
Abstract/Description: | Luteal steroidogenesis is critical to implantation and pregnancy maintenance in mammals. The role of androgen receptors (AR) in the progesterone (P4) producing luteal cells of porcine corpus luteum (CL) remains unexplored. The aim of the present study was to establish AR gene knock out (KO) porcine luteal cell culture system model by CRISPR/Cas9 genome editing technology and to study the downstream effects of AR gene deficiency on steroidogenic potential and viability of luteal cells. For this purpose, genomic cleavage detection assay, microscopy, RT-qPCR, ELISA, annexin, MTT, and viability assay complemented by bioinformatics analysis were employed. There was significant downregulation (p < 0.05) in the relative mRNA expression of steroidogenic marker genes STAR, CYP11A1, HSD3B1 in AR KO luteal cells as compared to the control group, which was further validated by the significant (p < 0.05) decrease in the P4 production. Significant decrease (p < 0.05) in relative viability on third passage were also observed. The relative mRNA expression of hypoxia related gene HIF1A was significantly (p < 0.05) downregulated in AR KO luteal cells. Protein-protein interaction analysis mapped AR to signaling pathways associated with luteal cell functionality. These findings suggests that AR gene functionality is critical to luteal cell steroidogenesis in porcine. |
Description: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Animal Biotechnology |
Journal Type: | Peer Reviewed |
NAAS Rating: | 8.28 |
Impact Factor: | 2.28 |
Volume No.: | doi.org/10.1080/10495398.2022.2079517 |
Page Number: | Not Available |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | https://doi.org/10.1080/10495398.2022.2079517 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/74560 |
Appears in Collections: | AS-NRCP-Publication |
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