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http://krishi.icar.gov.in/jspui/handle/123456789/1024| Title: | Genome–wide screening for novel, drought stress–responsive long non–coding RNAs in drought–stressed leaf transcriptome of drought tolerant and susceptible banana (Musa spp.) cultivars using Illumina high throughput sequencing. |
| Other Titles: | Not Available |
| Authors: | Muthusamy, M., Uma, S., Backiyarani, S. and Saraswathi, M. S. |
| Published/ Complete Date: | 2015-09-01 |
| Project Code: | Not Available |
| Keywords: | Banana, RNADrought stress-responsive, RNANatural antisense, RNAsmicro,RNA decoys, Genome-wide screening for novel, RNA |
| Publisher: | Springer Link |
| Citation: | 3 |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Long non-coding RNAs (LncRNAs) are one of the many layers of transcription in higher plants. LncRNAs are responsive to biotic and abiotic stresses and regulate genes. In our study, we have identified 905 novel lncRNAs from 8471 drought-responsive, novel transcripts of RNA-Seq reads from two banana cultivars, a drought-tolerant cv. ‘Saba’ (ABB) and -susceptible cv. ‘Grand Naine’ (AAA). Of these 905 lncRNAs, 75 (8.3 %) transcripts were natural antisense RNAs (NATs) and 2 transcripts identified as precursors of microRNA-miR156 and miR166. Among the 905 identified lncRNAs, 216, 150 and 279, 164 lncRNAs were induced and reduced to drought stress, respectively, in tolerant and susceptible in comparison to their equivalent controls. The remaining 22 lncRNA of tolerant cultivars was not regulated by drought stress. Of the 882 drought-responsive lncRNAs, 44 new lncRNAs were identified as induced. Musa lncRNAs were unevenly distributed in 11 chromosomes of Musa acuminata and no lncRNAs were found in chromosome-9 of drought-tolerant cultivar. The average lengths of lncRNAs were 683 nucleotides (nt). Drought-responsive differential expression of lncRNAs was found between +8.11585- and −4.04311-fold. Around 7.9 % of the identified lncRNAs were decoys of 85 conserved microRNAs. These findings will lay a basic platform for effective strategic planning of developing drought-resilient crop varieties. |
| Description: | Bananas are considered as an important food security crop, providing staple food for millions of people, especially third world countries. Banana crop productivity was severely affected by drought stress. Drought-induced yield losses were documented to be around 20–65 % in bananas, especially bananas with AAA genome of east Africa (Van Asten et al. 2010). Vast research efforts were made earlier to identify the QTL/network of genes responsible for drought stress tolerance. Till now, many layers of regulatory elements such as protein kinases, stress-induced expression of several protein-coding genes, transcription factors, small regulatory RNAs such as microRNAs, snoRNAs, siRNAs and several unknown factors have been identified (Ponting et al. 2009; Liu et al. 2012; Kornienko et al. 2013). However, still many factors contributing toward stress response are largely not known. Functional long non-coding RNAs in drought stress response have been reported in model plants (Liu et al. 2012; Zhang et al. 2014; Shuai et al. 2014). These lncRNAs are crucial gene transcription regulators in plant response to biotic and abiotic stress (Xin et al. 2011; Zhang et al. 2013). LncRNAs also play a major role in plant developmental processes (Amor et al. 2009). |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Plant Biotechnology Reports |
| NAAS Rating: | 7.46 |
| Volume No.: | 9 (5) |
| Page Number: | 279–286. |
| Name of the Division/Regional Station: | Horticulture |
| Source, DOI or any other URL: | http://link.springer.com/article/10.1007/s11816-015-0363-6 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/1024 |
| Appears in Collections: | HS-NRCB-Publication |
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| File | Description | Size | Format | |
|---|---|---|---|---|
| art%3A10.1007%2Fs11816-015-0363-6.pdf | 675.15 kB | Adobe PDF | View/Open |
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