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http://krishi.icar.gov.in/jspui/handle/123456789/10289
Title: | Sequence analysis of hexon gene of the Indian isolates of EDS-76 virus of Chicken and Japanese Quails (Coturnix coturnix japonica) |
Other Titles: | Not Available |
Authors: | Susitha Rajkumar B.B. Dash |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Central Coastal Agricultural Research Institute ICAR::Indian Veterinary Research Institute |
Published/ Complete Date: | 2017-04-02 |
Project Code: | Not Available |
Keywords: | Chicken EDS-76 virus Quail EDS-76 virus hexon gene sequencing Phylogenetic analysis |
Publisher: | International Society for Science Environment and Technology |
Citation: | Susitha, R., & Dash, B. B. SEQUENCE ANALYSIS OF HEXON GENE OF THE INDIAN ISOLATES OF EDS-76 VIRUS OF CHICKEN AND JAPANESE QUAILS (COTURNIX COTURNIX JAPONICA). |
Series/Report no.: | Not Available; |
Abstract/Description: | The hexon genes of Indian isolates of EDS-76 virus, comprising of two chicken isolates (CEDS-1& CEDS-2) and one quail isolate (QEDS) and the reference strain BC-14 were amplified, targeting the variable region (L1 to L4). The 1901bp PCR product was cloned and sequenced. The nucleotide and the amino acid sequences were analysed in comparison to the published sequences of the European EDS-76 isolate (EEU) and Chinese isolate of quail origin (QEC), which revealed the differences of 0.1% in nucleotide and 0.2% in amino acid sequences existing amongst the Indian isolates and with that of the reference strain BC-14. The CEDS-1 was showing more variations from other two Indian isolates (CEDS-2 and QEDS) and the reference strain that is 0.4% in nucleotide and 0.6% in amino acid sequences. The Indian isolates and the BC-14 strain had shown slight variation from that of the European chicken isolates and showing more than 5% variation from Chinese quail isolate. The BC-14 and EEU viruses were found of the same origin with 99.9% similarity. All the Indian EDS-76 virus isolates including the quail isolate had the similar lineage where as the Chinese quail isolate had a separate unique lineage as observed by Phylogenetic studies. |
Description: | Not Available |
ISSN: | 2277-663X |
Type(s) of content: | Research Paper |
Sponsors: | ICAR |
Language: | English |
Name of Journal: | International Journal of Science, Environment and Technology |
NAAS Rating: | Not Available |
Volume No.: | 6 |
Page Number: | 1236-1244 |
Name of the Division/Regional Station: | Animal Sciences |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/10289 |
Appears in Collections: | NRM-CCARI-Publication |
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