Molecular Characterization of the Recombinant Inbred Line Population Derived from a soja and max Soybean Cross

Abstract

Soybean is the most important oilseed crop in the world due to its high oil (20%) and protein (40%) content. Productivity of soybean in India is low. Poor genetic variability in soybean is an important factor of low yield. Wide hybridization was attempted between cultivated genotype DS9712 and wild type accession DC2008-1. Isolating from the segregating population in F2:6 generation, 206 RILs were developed and tested for two consecutive years i.e. kharif 2014 and 2015. Enormous variability was observed among the RILs for phenotypic traits including yield. Genetic polymorphism was studied with 317 SSR markers, out of which 206 were polymorphic (~65% polymorphism). Distribution of the polymorphic markers was not uniform on the chromosomes. Tri-nucleotide repeat motif SSR markers were more polymorphic than others. About 8 markers showed distorted segregation. Linkage map was constructed with 194 markers. Total length of the map was 1823.71cM with an average marker spacing of 9.21cM. The RILs and the molecular map produced in the study will be suitable for mapping and analyzing QTLs for yield and other related traits in soybean