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http://krishi.icar.gov.in/jspui/handle/123456789/11590| Title: | Transformation of indica rice through particle bombardment - factors influencing transient expression and selection |
| Other Titles: | Not Available |
| Authors: | KBRS Visarada NP Sarma |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Indian Institute of Millets Research ICAR::Indian Institute of Rice Research |
| Published/ Complete Date: | 2004-01-01 |
| Project Code: | Not Available |
| Keywords: | embryogenic calli regeneration selection |
| Publisher: | Not Available |
| Citation: | Visarada KBRS, arma NP. 2004. Transformation of indica rice through particle bombardment: factors influencing transient expression and selection. Biologia Plantarum 48 25-31 |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Embryogenic, friable and small (ca. 3 mm) calli showed optimum gus expression and were best suited for selection during genetic transformation of rice through particle bombardment. Through prolonged culture of mature seeds on original callus induction medium, this type of calli could be produced in large numbers across several elite rice genotypes. To minimize the non-transformed escapes 50 mg dm−3 hygromycin and 8 mg dm−3 glufosinate ammonium were found to be critical during selection. Addition of selection marker during regeneration was essential. Regular and frequent (every 15 d) transfer of calli to fresh selection medium for three cycles was also important. A simple and economic procedure for screening large number of putative resistant plants was described. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Journal |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Biologia Plantarum |
| NAAS Rating: | 7.6 |
| Volume No.: | 48 |
| Page Number: | 25-31 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | https://doi.org/10.1023/B:BIOP.0000024271.38723.a6 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/11590 |
| Appears in Collections: | CS-IIMilletsR-Publication |
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