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http://krishi.icar.gov.in/jspui/handle/123456789/13161Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Mishra A | en_US |
| dc.contributor.author | Reddy IJ | en_US |
| dc.contributor.author | Gupta PSP | en_US |
| dc.contributor.author | Mondal S | en_US |
| dc.date.accessioned | 2018-11-24T10:59:55Z | - |
| dc.date.available | 2018-11-24T10:59:55Z | - |
| dc.date.issued | 2016-09-30 | - |
| dc.identifier.citation | Mishra A, Reddy IJ, Gupta PSP and Mondal S. 2016. Developmental regulation and modulation of apoptotic genes expression in sheep oocytes and embryos cultured in vitro with L-carnitine. Reproduction in Domestic Animals, 51:1020-1029. | en_US |
| dc.identifier.issn | 1439-0531 | - |
| dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/13161 | - |
| dc.description | Not Available | en_US |
| dc.description.abstract | The objective of this study was to find out the impact of L-carnitine (10 mM) on developmental regulation of preimplantation sheep embryos cultured in vitro when supplemented in maturation medium and post-fertilization medium separately. Subsequent objective was to observe the L-carnitine-mediated alteration in expression of apoptotic genes (Bcl2, Bax, Casp3 and PCNA) in sheep oocytes and developing embryos produced in vitro. Oocytes matured with L-carnitine showed significantly (p < .05) higher cleavage (67.23% vs 43.12%), morula (47.65% vs 28.58%) and blastocysts (32.12% vs 13.24%) percentage as compared to presumptive zygotes cultured with L-carnitine during post-fertilization period. So it is suggested to use L-carnitine during maturation than post-fertilization period. Antiapoptotic and proliferative effects of L-carnitine were confirmed by inducing culture medium with actinomycin D (apoptotic agent) and TNFα (antiproliferative agent), respectively, with and without L-carnitine. Oocytes and embryos cultured with actinomycin D and TNFα showed developmental arrest with significant (p < .05) decrease in morula and blastocysts percentage but supplementation of L-carnitine to actinomycin D and TNFα induced culture medium showed similar result as that of control. L-carnitine supplementation during IVM significantly (p < .05) upregulated the expression of Bcl2 and PCNA genes in majority of the developmental stages. Although L-carnitine upregulated the expression of Bax in initial developmental stages but downregulated at latter part, whereas the expression of Casp3 was upregulated upto 16-cell stage but after that there was no difference in expression. Expression of GAPDH gene was not affected by L-carnitine supplementation. In conclusion, L-carnitine acted as an antiapoptotic and proliferative compound during embryo development and supplementation of L-carnitine during IVM altered the expression of apoptotic genes in the developmental stages of embryos. | en_US |
| dc.description.sponsorship | Not Available | en_US |
| dc.language.iso | English | en_US |
| dc.publisher | John Wiley & Sons | en_US |
| dc.relation.ispartofseries | Not Available; | - |
| dc.subject | apoptotic genes expression | en_US |
| dc.subject | sheep oocytes | en_US |
| dc.subject | L-carnitine | en_US |
| dc.title | Developmental regulation and modulation of apoptotic genes expression in sheep oocytes and embryos cultured in vitro with L-carnitine | en_US |
| dc.title.alternative | Not Available | en_US |
| dc.type | Research Paper | en_US |
| dc.publication.projectcode | Not Available | en_US |
| dc.publication.journalname | Reporduction in Domestic Animals | en_US |
| dc.publication.volumeno | 51 | en_US |
| dc.publication.pagenumber | 1020-1029 | en_US |
| dc.publication.divisionUnit | A.P. DIV | en_US |
| dc.publication.sourceUrl | DOI: 10.1111/rda.12789 | en_US |
| dc.publication.authorAffiliation | ICAR-NIANP | en_US |
| dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
| dc.publication.naasrating | 7.64 | en_US |
| Appears in Collections: | AS-NIANP-Publication | |
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