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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/13647
Title: | Effect of immune gene silencing in WSSV infected tiger shrimp Penaeus monodon |
Other Titles: | Not Available |
Authors: | M.S. Shekhar A. Gomathi N.K. Dubey K. Vinaya Kumar KK.Vijayan |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR-Central Institute of Brackishwater Aquaculture |
Published/ Complete Date: | 2017-01-19 |
Project Code: | Not Available |
Keywords: | Differentially expressed genes, RNAi, Immune genes, Gene silencing, Penaeus monodon, WSSV |
Publisher: | CIBA |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | White spot syndrome virus, continues to cause huge economic loss to aquaculture industry. In the absence of effective therapeutics to control WSSV, it is important to understand the host pathogen interaction at the molecular level. Suppression subtractive hybridization (SSH) cDNA library was constructed which led to identification of several differentially expressed genes in response to WSSV infection in Penaeus monodon. The genes expressed in SSH cDNA library of shrimp gill and gut tissues belonged to a wide range of biological functions. The three differentially expressed genes, Single von Willebrand factor type C domain protein (pmSVC), P53 protein gene (pmP53) and ADP ribosylation factor (pmArf) were up-regulated against WSSV infection and were further characterized by gene silencing to study the role of these shrimp immune genes on WSSV multiplication. The sequence-specific knock down of pmSVC, pmP53 and pmArf using the dsRNA revealed that in pmSVC-dsRNA inoculated shrimps WSSV replication was more with increased viral copy numbers when compared with pmP53-dsRNA and pmArf -dsRNA inoculated shrimps. The varied response of immune genes to WSSV infection, indicated that host genes may either inhibit virus replication to some extent or might act as a target to facilitate viral pathogenesis. |
Description: | Not Available |
ISSN: | 1050-4648 |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Fish and Shellfish Immunology |
NAAS Rating: | 6 |
Volume No.: | 70 |
Page Number: | 252-259 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/13647 |
Appears in Collections: | FS-CIBA-Publication |
Files in This Item:
File | Description | Size | Format | |
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immune gene silencing.pdf | 832.01 kB | Adobe PDF | View/Open |
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