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http://krishi.icar.gov.in/jspui/handle/123456789/13724| Title: | Comparative gene expression profiling during in vitro regeneration in two coconut cultivars |
| Other Titles: | Not Available |
| Authors: | Udyawar Bhavyashree Kaitheri Edathil Rachana Kooliyat Lakshmi Jayaraj Muliyar Krishna Rajesh Karun Anitha |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Central Plantation Crops Research Institute |
| Published/ Complete Date: | 2016-06-01 |
| Project Code: | Not Available |
| Keywords: | Coconut Gene expression Recalcitrance Shoot meristem Somatic embryogenesis |
| Publisher: | Not Available |
| Citation: | Emirates Journal of Food and Agriculture. 2016. 28(8): 572-580 |
| Series/Report no.: | Not Available; |
| Abstract/Description: | A comparative study of gene expression patterns during different stages of in vitro regeneration in two coconut cultivars viz. WCT (West Coast Tall) and COD (Chowghat Orange Dwarf) was carried out in this study. Calli were obtained from shoot meristem explants and expression patterns of eight genes at different development stages in the two cultivars were analyzed using RT-qPCR. The results revealed the enhanced expression of PKL, SERK and WUS in embryogenic calli compared to non-embryogenic calli. High expression of GLP, ECP and GST could be observed in normal somatic embryos compared to abnormal somatic embryos. Enhanced expression of ECP, LEAFY, GLP and WRKY could be observed in normal meristemoids compared to aberrant meristemoids. When the two cultivars were compared, higher somatic embryogenesis was noticed in WCT as compared to COD. Embryogenic calli of WCT showed higher expression of SERK, PKL and WUS compared to COD. Somatic embryos of WCT showed high expression of GLP and GST compared to COD, where as for ECP gene, higher expression was observed in WCT compared to COD. In cases of WRKY and LEC, higher levels of expression were observed in WCT meristemoids when compared to COD. The results revealed signifi cant differences in regeneration potential and gene expression patterns in the two coconut cultivars suggesting genotypic differences of cultivars to in vitro culture. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Article |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Emirates Journal of Food and Agriculture |
| NAAS Rating: | 7.01 |
| Volume No.: | 28 |
| Page Number: | 572-580 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/13724 |
| Appears in Collections: | HS-CPCRI-Publication |
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