Antibiotic Resistance of Escherichia coli isolated from Seafood of Veraval, Gujarat to third Generation Cephalosporins

Abstract

A total of 238 seafood samples were collected from fish market and fish processing industries of Veraval region, Gujarat during 2012 to 2015. Four 3rd generation cephalosporins; Ceftazidime (CAZ) 30 μg, Ceftizoxime (CZX) 30 μg, Cefotaxime (CTX) 30 μg and Ceftriazone (CTR) 30 μg (HiMedia, Mumbai) were tested by the disk diffusion method (Bauer et al., 1966) on Mueller-Hinton agar (Oxoid, UK) with 0.1 ml of E. coli equivalent to 0.5 McFarland standards in accordance with CLSI guidelines (CLSI, 2014) of antimicrobial concentration and incubated for 18-24 h at 37°C. The results were interpreted as described by CLSI, (2014). The E. coli isolates ATCC 25922 was used as control. E. coli was detected in 11.76% of the samples with counts ranging from 101 to 2x102 cfu g-1. Similar to the present findings, Sivaraman et al. (2014) reported high count of E. coli in seafood from Gujarat ranging from 0.5 to 2.0 x 103 cfug-1 with the incidence rate of 53.19% and suggested, the possibility of faecalor sewage as the source of contamination.