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http://krishi.icar.gov.in/jspui/handle/123456789/15021| Title: | Molecular characterization, structure prediction and insilico analysis of hydrocarbon degrading surfactin synthetase from marine sponge-associated Bacillus licheniformis NIOT-06 |
| Other Titles: | Not Available |
| Authors: | Anburajan, L. Meena, B. Rangamaran, V.R Toms C. Joseph Vinithkumar, N.V. Gopa, D Kirubagaran, R |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Central Institute of Fisheries Technology Andaman and Nicobar Centre for Ocean Science and Technology, Earth System Sciences Organization-National Institute of Ocean Technology (ESSO-NIOT), Andaman and Nicobar Islands, India Ocean Science and Technology for Islands Group, ESSO-NIOT, Ministry of Earth Sciences, Government of India, Tamil Nadu, India |
| Published/ Complete Date: | 2016-12 |
| Project Code: | Not Available |
| Keywords: | Marine sponge Bacillus licheniforms NIOT-06 Biosurfactant gene Surfactin synthetase Recombinant biosurfactant |
| Publisher: | Elsevier |
| Citation: | Anburajan, L., Meena, B., Rangamaran, V.R., Toms C. Joseph, Vinithkumar, N.V., Gopa, D. and Kirubagaran, R. (2016) - Molecular characterization, structure prediction and in silico analysis of hydrocarbon degrading surfactin synthetase from marine sponge-associated Bacillus licheniformis. Gene Reports, 5: 40-44. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | An open reading frame encoding the surfactin synthetase gene (srfA) was cloned from the Bacillus licheniformis NIOT-06 genome. An expression plasmid containing the srfA operon was introduced into Escherichia coli M15 cells and the recombinant biosurfactant was purified and quantified. Purified recombinant surfactin revealed excellent emulsification activity. HPLC fraction of the purified recombinant surfactin revealed significant surface tension reducing property. Secondary structure of the surfactin synthetase enzyme was predicted to have alpha-helical structure. Three dimensional structure model of surfactin synthase was found to be 0.243 Å units. |
| Description: | Not Available |
| ISSN: | 2452-0144 |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Gene Reports |
| Volume No.: | 5 |
| Page Number: | 40-44 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | https://doi.org/10.1016/j.genrep.2016.08.006 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/15021 |
| Appears in Collections: | FS-CIFT-Publication |
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