KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/15078
Title: | Heterologous expression, purification, and phylogenetic analysis of oil-degrading biosurfactant biosynthesis genes from the marine sponge-associated Bacillus licheniformis NIOT-06 |
Other Titles: | Not Available |
Authors: | Anburajan, L. Meena, B. Raghavan, R.V. Sridhar, D. Toms C. Joseph Vinithkumar, N.V Dharani, G. Dheenan, G.P.S. Kirubagaran, R. |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | Andaman and Nicobar Centre for Ocean Science and TechnologyEarth System Sciences Organization-National Institute of Ocean Technology (ESSO-NIOT)Port Blair Ocean Science and Technology for Islands GroupESSO-NIOT, Ministry of Earth Sciences, Government of India ICAR::Central Institute of Fisheries Technology |
Published/ Complete Date: | 2014-11-15 |
Project Code: | Not Available |
Keywords: | Bacillus licheniformis NIOT-06 Marine sponge Biosurfactant Biosurfactant biosynthesis genes Biosurfactant biosynthesis genes |
Publisher: | Springer Berlin Heidelberg |
Citation: | Anbu Rajan, L., Meena, L., Raghavan, R.V., Sridhar, D., Toms C. Joseph, Vinithkumar, N.V., Dharani, G., Dheenan, G.P.S. and Kirubagaran, R. (2015) – Heterologous expression, purifcation and phylogenetic analysis of oil-degrading biosurfactant biosysnthesis genes from the marine sponge-associated Bacillus licheniformis NIOT-06, Bioprocess Biosyst. Engg., doi 10.1007/200449-015-1359-x |
Series/Report no.: | Not Available; |
Abstract/Description: | Surfactin is a lipopeptide, composed of one β-hydroxy fatty acid, a long fatty acid moiety, and seven amino acids. In this study, the biosurfactant biosynthesis genes; 4′-pantetheinyl transferase (sfp), phosphopantetheinyl transferase (sfpO), and surfactin synthetase (srfA) have been characterized from the marine sponge-associated Bacillus licheniformis NIOT-06 from the Andaman and Nicobar Islands. The purified recombinant biosurfactant revealed excellent emulsification activity with crude oil and kerosene. Reverse-phase high-performance liquid chromatography resolved the purified recombinant biosurfactant into several fractions and one of which had significant surface tension reducing property. Fourier transform infrared spectroscopy spectrum also revealed the presence of C–N–N, alkenes, and N–H as the functional groups, and a similar overlapping pattern was observed with that of standard lipopeptide surfactin. The diversity and phylogeny of sfp, sfpO, and srfA gene sequences were compared with other eubacteria. The sfp, sfpO, and srfA gene sequences obtained from Bacillus licheniformis NIOT-06 were diverse and appeared to be partially conserved when compared with the GenBank reported sequences of several eubacteria. |
Description: | Not Available |
ISSN: | 1615-7591(Print) 1615-7605 (Online) |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Bioprocess and Biosystems Engineering |
NAAS Rating: | 8.42 |
Volume No.: | 38 |
Page Number: | 1009–1018 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | https://doi.org/10.1007/s00449-015-1359-x |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/15078 |
Appears in Collections: | FS-CIFT-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.