Biochemical Profiles in Freezable and Non-Freezable Ejaculates of Mithun (Bos frontalis) Semen

Abstract

The present study was undertaken to measure the biochemical profiles of freezable and nonfreezable ejaculates of Mithun. Fifty ejaculates (twenty five ejaculates each from freezable and non- freezable ones) were collected from matured Mithun bulls. Biochemical profiles viz., alkaline phosphatase (ALP), acid phosphatase (ACP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactic acid dehydrogenase (LDH), calcium (Ca), magnesium (Mg), zinc (Zn), chloride (Cl), citric acid (CA), total seminal plasma protein (TSPP), inorganic phosphorous (IP), fructose and cholesterol concentration in sperm were estimated. The result revealed that these parameters varied significantly (P< 0.05) between the freezable and non-freezable ejaculates. Freezable ejaculates has significantly (P< 0.05) higher ALP, ACP, LDH, Ca, Mg, Zn, citric acid, TSPP, IP, fructose and cholesterol concentration and significantly (P< 0.05) lower Cl concentration than the nonfreezable ejaculates. There was a correlation among the biochemicals such as ALP, ACP, LDH, Ca, Mg, Zn, CA, TSPP, IP, fructose, cholesterol and these biochemicals were negatively correlated with AST, ALT and Cl in both freezable and non-freezable ejaculates. It was concluded that most of the biochemical parameters were higher in freezable ejaculates than the non-freezable ejaculates, indicates that freezable ejaculates have has structural stability than the non-freezable ones that leads to freezable sperm has higher functional structures to move faster and in forward direction.