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http://krishi.icar.gov.in/jspui/handle/123456789/1766| Title: | Embryo rescue and plant regeneration in banana (Musa spp.). |
| Authors: | Uma S, S. Lakshmi , M.S. Saraswathi, A. Akbar and M.M. Mustaffa. |
| Published/ Complete Date: | 2010-10-17 |
| Keywords: | Banana, Embryo maturity, In vitro culture, Zygotic embryo, Callus, Regeneration |
| Publisher: | Springer Link |
| Citation: | 19 |
| Abstract/Description: | An efficient regeneration protocol for zygotic embryos at varying maturity stages was developed for wild banana (Pisang Jajee (AA)). Embryo ontogeny was studied to determine the best maturity stage for embryo rescue, suitable media and culture conditions (light and dark) for germination and regeneration. The conversion of endosperm from transparent fluid into a semi-solid state was followed by visible embryo development, which commenced only after 70% embryo maturity. Zygotic embryos of Pisang Jajee at different maturity levels were excised and cultured on medium fortified with different concentrations of 6-benzyl adenine (BA) and indole acetic acid (IAA). Zygotic embryos produced callus or plantlets 25 days after initiation. The frequency of callus induction was greater in immature embryos irrespective of the media composition and decreased with increasing maturity. Fully matured embryos regenerated directly into plantlets without producing callus. Immature embryos required medium supplemented with plant growth regulators (PGRs) for successful regeneration. Although the culture conditions had no influence, dark conditions favoured callus induction and plant regeneration. |
| Description: | Bananas and plantains are giant monocotyledonous perennial herbs, belonging to the order Zingiberales (Scitamineae) and family Musaceae. Banana is one of the few fruit crops in which all of the cultivated varieties are human selections. Its recalcitrance to improvement through breeding is mainly due to parthenocarpy and male and/or female sterility among the most cultivated varieties. Diverse genomic constitutions like AA, AB, AAB, ABB etc. with varied ploidy levels (2×, 3× and 4×) have aggravated the problem of improvement through classical breeding. These incompatibility problems have manifested into improper seed formation, poor embryo development, endosperm malformation etc. The success of a breeding programme depends on compatibility among the parents and the production of a good number of fertile seeds. More often, the banana seeds, although complete in terms of embryo, endosperm and seed coat, fail to germinate due to various reasons (Chin 1996). Under such circumstances, embryo culture becomes vital. Failure of embryo germination is attributed to the activation of growth inhibitors at the advanced maturity stage of seed development, leading to seed coat dormancy (Le Page-Degivry and Garello 1973; Bewley and Black 1982; Fenner 1985). Therefore, it becomes essential to rescue the embryos before maturity in order to salvage the maximum number of hybrid progenies in a breeding programme. |
| Type(s) of content: | Research Paper |
| Language: | English |
| Name of Journal: | Plant Cell Tissue and Organ Culture |
| NAAS Rating: | 8.2 |
| Volume No.: | 105(1) |
| Page Number: | 105–111 |
| Name of the Division/Regional Station: | Horticulture |
| Source, DOI or any other URL: | http://link.springer.com/article/10.1007/s11240-010-9847-9 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/1766 |
| Appears in Collections: | HS-NRCB-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| art%3A10.1007%2Fs11240-010-9847-9.pdf | 606.97 kB | Adobe PDF | View/Open |
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