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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/19842
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ghosh KK | en_US |
dc.contributor.author | Prakash A | en_US |
dc.contributor.author | Shrivastav P | en_US |
dc.contributor.author | Balamurugan V | en_US |
dc.contributor.author | Kumar M. | en_US |
dc.date.accessioned | 2019-05-25T03:15:12Z | - |
dc.date.available | 2019-05-25T03:15:12Z | - |
dc.date.issued | 2018-07-03 | - |
dc.identifier.citation | Not Available | en_US |
dc.identifier.issn | 2996-9088 | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/19842 | - |
dc.description | Not Available | en_US |
dc.description.abstract | The outer membrane proteins of the pathogen are targeted to understand host-pathogen interactions and are central to the development of diagnostics. We report that Leptospira interrogans serovar Copenhageni strain Fiocruz L1-130 contains a gene LIC13341 that encodes a conserved outer membrane/periplasmic lipoprotein. The gene LIC13341 was cloned into expression vector pET28a and the recombinant LIC13341 (r-LIC13341) protein was purified from Escherichia coli BL21 (DE3) using affinity chromatography. The secondary structure of the purified r-LIC13341 protein featured a typical β-strand when observed by circular dichroism spectroscopy. Immunoblotting using antibodies raised against r-LIC13341 in BALB/c mice can detect LIC13341 expression in the Leptospira lysates and suggested that antigen LIC13341 is immunogenic. Phase separation and protease assays determined that LIC13341 is a surface-exposed outer membrane protein of Leptospira. The r-LIC13341 can bind to a wide spectrum of host extracellular matrices (ECMs). The specific adherence of Leptospira to laminin and hyaluronic acid of the ECM was competitively inhibited in the presence of r-LIC13341. The enzyme-linked immunosorbent assay and immunoblot performed using human or bovine leptospirosis serum (n=50) recognized r-LIC13341, suggesting that LIC13341 is expressed in diverse hosts during Leptospira infection. Thus, the present finding suggests that the Leptospira LIC13341 antigen is a versatile outer membrane adhesin of diagnostic importance. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Microbiology society | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | ELISA | en_US |
dc.subject | Leptospira | en_US |
dc.subject | antibodies | en_US |
dc.subject | extracellular matrix component | en_US |
dc.subject | lipoprotein | en_US |
dc.subject | recombinant protein | en_US |
dc.title | Evaluation of a novel outer membrane surface-exposed protein, LIC13341 of Leptospira, as an adhesin and serodiagnostic candidate marker for leptospirosis. | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Microbiology | en_US |
dc.publication.volumeno | 164 | en_US |
dc.publication.pagenumber | 1023-1037 | en_US |
dc.publication.divisionUnit | Animal science | en_US |
dc.publication.sourceUrl | https://doi.org/10.1099/mic.0.000685 | en_US |
dc.publication.authorAffiliation | Indian Institute of Technology, Guwahati, Assam | en_US |
dc.publication.authorAffiliation | ICAR::National Institute of Veterinary Epidemiology and Disease Informatics | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.naasrating | 6.95 | en_US |
Appears in Collections: | AS-NIVEDI-Publication |
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