KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/20209
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | T. Sathish Kumar | en_US |
dc.contributor.author | Navaneeth Krishnan A | en_US |
dc.contributor.author | Joseph Sahaya Rajan J | en_US |
dc.contributor.author | M. Makesh | en_US |
dc.contributor.author | KP. Jithendran | en_US |
dc.contributor.author | S.V. Alavandi | en_US |
dc.contributor.author | KK.Vijayan | en_US |
dc.date.accessioned | 2019-06-12T07:01:45Z | - |
dc.date.available | 2019-06-12T07:01:45Z | - |
dc.date.issued | 2018-03-17 | - |
dc.identifier.citation | Not Available | en_US |
dc.identifier.uri | https://doi.org/10.1007/s00436-018-5828-4 | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/20209 | - |
dc.description | Not Available | en_US |
dc.description.abstract | The emerging microsporidian parasite Enterocytozoon hepatopenaei (EHP), the causative agent of hepatopancreatic microsporidiosis, has been widely reported in shrimp-farming countries. EHP infection can be detected by light microscopy observation of spores (1.7 × 1 μm) in stained hepatopancreas (HP) tissue smears, HP tissue sections, and fecal samples. EHP can also be detected by polymerase chain reaction (PCR) targeting the small subunit (SSU) ribosomal RNA (rRNA) gene or the spore wall protein gene (SWP). In this study, a rapid, sensitive, specific, and closed tube visual loop-mediated isothermal amplification (LAMP) protocol combined with FTA cards was developed for the diagnosis of EHP. LAMP primers were designed based on the SSU rRNA gene of EHP. The target sequence of EHP was amplified at constant temperature of 5 °C for 45 min and amplified LAMP products were visually detected in a closed tube system by using SYBR™ green I dye. Detection limit of this LAMP protocol was ten copies. Field and clinical applicability of this assay was evaluated using 162 field samples including 106 HP tissue samples and 56 fecal samples collected from shrimp farms. Out of 162 samples, EHP could be detected in 62 samples (47 HP samples and 15 fecal samples). When compared with SWP-PCR as the gold standard, this EHP LAMP assay had 95.31% sensitivity, 98.98% specificity, and a kappa value of 0.948. This simple, closed tube, clinically evaluated visual LAMP assay has great potential for diagnosing EHP at the farm level, particularly under low-resource circumstances. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Springer | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | EHP . LAMP . Closed tube LAMP . SYBR green I dye . FTA cards | en_US |
dc.title | Visual loop-mediated isothermal amplification (LAMP) for the rapid diagnosis of Enterocytozoon hepatopenaei (EHP) infection | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Article | en_US |
dc.publication.projectcode | CRP V & D | en_US |
dc.publication.journalname | Parasitology Research | en_US |
dc.publication.volumeno | 117(5) | en_US |
dc.publication.pagenumber | Not Available | en_US |
dc.publication.divisionUnit | AAHED | en_US |
dc.publication.sourceUrl | Not Availahttps://doi.org/10.1007/s00436-018-5828-4ble | en_US |
dc.publication.authorAffiliation | ICAR::Central Institute of Brackishwater Aquaculture | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.naasrating | 7.64 | en_US |
Appears in Collections: | FS-CIBA-Publication |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
EHP LAMP - Author's copy.pdf | 2.14 MB | Adobe PDF | View/Open |
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.