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http://krishi.icar.gov.in/jspui/handle/123456789/21488
Title: | Quantitative Analysis of Irigenin in the Different Species of Iris Plant by RP- HPLC and its Efficacy Against Different Plant Pathogens |
Other Titles: | Not Available |
Authors: | Sajad Hassan Wani Hilal Ahmad Bhat Javid Iqbal Mir Shahid Ali Akbar Sajad Un Nabi Desh Beer Singh Nazeer Ahmad |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Central Institute of Temperate Horticulture |
Published/ Complete Date: | 2017 |
Project Code: | Not Available |
Keywords: | Iris Plant Irigenin RP-HPLC Bio efficacy |
Publisher: | Pharmacognosy Network Worldwide |
Citation: | : Wani SH, Bhat HA, Mir JI, Akbar SA, Nabi Su, Singh DB, Ahmad N. Quantitative Analysis of Irigenin in the different Species of Iris Plant by RP- HPLC and its Efficacy against different Plant Pathogens. Pharmacog J. 2017;9(6)Suppl:s23-s27. |
Series/Report no.: | Not Available; |
Abstract/Description: | Irigenin belongs to family of isoflavonoids, isolated from Iris Plant of Kashmir Himalaya. A rapid and specific reverse phase high pressure chromatography (RP HPLC) method for quantitative analysis of irigenin in the different species of Iris Plant was developed. The samples were analyzed on RP-C18 e column (chromolith, 5µm, 4.6×100 mm). The HPLC system was operated at ambient temperature (±30c). The mobile phase consisted of methanol: water. The detecting wavelength at 260 nm and flow rate of 0.6 ml/min. The standard irigenin was diluted using the mobile phase at a known concentration of 1mg/ml; the sample was filtered through sample filter of 0.45 µ pore size. The filtrate was introduced on to a reverse phase analytical column. The content of irigenin in the different species of Iris Plant was determined. The HPLC showed an excellent performance in separating the irigenin in different species of Iris Plant. Furthermore, the antipathogenic activity. The test compound at each respective concentration was found to be statistically superior against scab. Furthermore, the test compound @ 5000 ppm proved significantly most effective by providing (82.49%) inhibition in the mycelia growth of apple scab. It was followed by fusarium (77.27%) at 5000 ppm. Lowest reduction in mycelia growth (65.78%) was recorded in marssonina and did not differ significantly from Alternaria (67.47%) at 5000 ppm. Furthermore, lowest inhibition of mycelia growth was recorded at 1000 ppm. Similar trend was recorded for rest of the pathogens i.e. highest reduction at 5000 ppm, lowest at 1000 ppm and at 2000, 3000 and 4000 ppm it ranges between the first two but increases with increase in concentration. From this study irigenin is potent compound which can be used for controlling the growth of respective pathogens. |
Description: | Not Available |
ISSN: | 09753575 |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Pharmacognocy Journal |
Volume No.: | 9(6) |
Page Number: | 23-27 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/21488 |
Appears in Collections: | HS-CITH-Publication |
Files in This Item:
File | Description | Size | Format | |
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SH Wani.pdf | 495.76 kB | Adobe PDF | View/Open |
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