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http://krishi.icar.gov.in/jspui/handle/123456789/23098
Title: | A GFP-based bacterial biosensor with chromosomally integrated sensing cassette for quantitative detection of Hg (II) in environment |
Other Titles: | Not Available |
Authors: | Priyadarshi, H. Alam, A., Gireesh-Babu, P., Das, R., Kishore, Kumar, S. Chaudhari, A |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Central Inland Fisheries Research Institute |
Published/ Complete Date: | 2012-05-01 |
Project Code: | Not Available |
Keywords: | attP/attB integrationE. coli biosensorGFPmercury |
Publisher: | Not Available |
Citation: | Priyadarshi, H., Alam, A., Gireesh-Babu, P., Das, R., Kishore, P., Kumar, S. and Chaudhari, A. 2012. A GFP-based bacterial biosensor with chromosomally integrated sensing cassette for quantitative detection of Hg (II) in environment. Journal of Environmental Sciences, 24(5): 963–968. |
Series/Report no.: | Not Available; |
Abstract/Description: | A mercury biosensor was constructed by integrating biosensor genetic elements into E. coli JM109 chromosome in a single copy number, using the attP/attB recombination mechanism of γ phage. The genetic elements used include a regulatory protein gene (merR) along with operator/promoter (O/P) derived from the mercury resistance operon from pDU1358 plasmid of Serratia marcescens. The expression of reporter gene gfp is also controlled by merR/O/P. Integration of the construct into the chromosome was done to increase the stability and precision of the biosensor. This biosensor could detect Hg(II) ions in the concentration range of 100-1700 nmol/L, and manifest the result as the expression of GFP. The GFP expression was significantly different (P ⩽ 0.05) for each concentration of inducing Hg(II) ions in the detection range, which reduces the chances of misinterpretation of results. A model using regression method was also derived for the quantification of the concentration of Hg(II) in water samples. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Journal of Environmental Sciences |
NAAS Rating: | 10.3 |
Volume No.: | 24(5) |
Page Number: | 963–968. |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | https://doi.org/10.1016/S1001-0742(11)60820-6 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/23098 |
Appears in Collections: | FS-CIFRI-Publication |
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