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http://krishi.icar.gov.in/jspui/handle/123456789/24253| Title: | Differentiation of Phytophthora species associated with plantation crops using PCR and high-resolution melting curve analysis |
| Other Titles: | Not Available |
| Authors: | Prathibha, V.H Vinayaka Hegde Sharadraj, K.M Rajesh, M.K Rachana, K.E Chowdappa, P |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Central Plantation Crops Research Institute |
| Published/ Complete Date: | 2018-01-01 |
| Project Code: | Not Available |
| Keywords: | Coconut Arecanut palm Phytophthora HRM analysis Rapid detection PCR |
| Publisher: | Not Available |
| Citation: | Journal of Plant Pathology,100(2):233-240 |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Diseases caused by closely related species of Phytophthora such as P. palmivora, P. nicotianae, P. capsici, P. meadii, P. citrophthora and P. colocasiae are major production constraints of plantation crops in India. A reliable method for the rapid identification of these Phytophthora species is required for production of disease-free planting material and promote exports. A specific PCR assay with primer pairs designed from the ITS region effectively differentiated Phytophthora species belonging to different clades viz., P. palmivora, P. nicotianae and P. capsici. In order to differentiate closely related species (P. meadii, P. citrophthora and P. colocasiae), a high-resolution melting (HRM) curve analysis, using primers designed based on single nucleotide differences in A/T and C/A alleles was developed and validated. HRM curve analysis proved to be a fast and accurate technique for differentiation of closely related species of Phytophthora. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Article |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Journal of Plant Pathology |
| NAAS Rating: | 7.15 |
| Volume No.: | 100(2) |
| Page Number: | 233-240 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/24253 |
| Appears in Collections: | HS-CPCRI-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| differentiation.pdf | 1.04 MB | Adobe PDF | View/Open |
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