Scrotal infrared digital thermography predicts effects of thermal stress on buffalo (Bubalus bubalis) semen.

Abstract

This aim of this study was to assess the relationship, if any, of scrotal surface temperature gradient (SSTG), scrotal circumference (SC) and testicular covering thickness (TCT) with semen quality in Murrah buffalo breeding bulls. For this, buffalo breeding bulls (n = 130) were selected from four different semen centres. The ejaculates of each bull were evaluated for ejaculate volume (EV), mass motility (MM); concentration (SPC), motility (SM), viability and abnormalities (SA). The SSTG, SC and TCT of individual bulls were quantified using digital infrared thermography, measuring tape and ultrasonography, respectively. The bulls were divided into three groups on the basis of SSTG (≤ 4 °C, 4.1 to 6.4 °C and ≥ 6.5 °C), and SC (< 31, 31 to 35 and > 35 cm) and into two groups on the basis of TCT (5 to 7.2 and 7.4 to 10.4 mm). Results indicated the bulls with a larger temperature gradient and larger SC produced greater quality semen than those with a lesser temperature gradient. The MM (P < 0.01) and SPC (P < 0.05) varied among the groups along with SSTG. Among the SC groups the EV, MM, SPC and SM (P < 0.01), and viability (P < 0.05) varied as did the SC. The bulls with a lesser TCT had a lesser SA (P < 0.05) as compared to the group with the greater TCT. Buffalo bulls having a greater SSTG, SC and lesser TCT produced semen of greater quality and these variables may be used as criteria for breeding soundness evaluation