Molecular signature of selective microRNAs in Cyprinus carpio (Linnaeus 1758):a computational approach

Abstract

MicroRNAs (miRNAs) are small single stranded non-coding RNAs that performed significant role in post-transcriptional regulation of target gene product. They are typically conserved among the organisms and also involved in multiple biological processes by degrading the targeted mRNAs by suppression or destabilization. Their conserved nature in various organisms provide a good source of miRNA identification and characterization using comparative genomic approaches through the bio-computational tools. The present work highlighted about common carp’s (Cyprinus carpio) conserved miRNAs which were identified and characterized using some bioinformatical strategies based on miRBase, Expressed Sequence Tag (EST) and Genomic Survey Sequence (GSS) databases. Using web based tools total 10 selected miRNAs belongs to 10 different families were illustrated and functionally categorized in Cyprinus carpio. Which have elemental role in regulation and expression of gene-protein interaction specially focused in biological processes of carp. Through real time application of mature miRNA sequences as probe the effective targets for selected common carp miRNAs were detected and catagorised using local BLAST online program and miRBase software. Imperative miRNAs of Cyprinus carpio are indentified via miRBase database and cautiously characterized with the associated gene encoded target protein. Those proteins having a key regulatory role in cellular signal transduction, transcription factor and associated biomolecules responsible for the metabolism, growth and development of carp skeletal muscle as well as preconditioning of skeletal myoblasts components. These selected miRNAs and their targets in common carp may enhance the better understanding and knowhow of miRNAs as superior role in regulating the metabolism, growth and developmental factors of Cyprinus carpio.