An integrated system for simultaneous casting of multi-polyacrylamide gels with varied concentrations.

Abstract

The ever growing advancements in the protein analysis of any cell or tissue using different variants of polyacrylamide gel electrophoresis and associated basic techniques offers its widespread application in almost all molecular biology laboratories. On the other hand, casting of several gels in parallel is a cumbersome task but is often an essential situation. A multi-gel casting apparatus for simultaneous casting of homogeneous concentration and gradient concentration sodium dodecyl sulphate- polyacrylamide gel electrophoresis (SDS-PAGE) gel was described here. The efficiency of this newly modified apparatus was significantly improved by the flexibility of using the top pouring and bottom pouring mechanism at the same time along with the siphoning of used solution from polymerizing bottom gel surface which makes it suitable for all types of vertical polyacrylamide gels. This apparatus reliably produces different kinds of slab gels in multiple numbers with each gel having similar physical characteristics in its type and provides desired results comparable to the gels made by commercial sources.